PPT-CSE182 CSE182-L12 Mass Spectrometry

Peptide identification CSE182 General isotope computation Definition Let p ia be the abundance of the isotope with mass i Da above the least mass Ex P 0C abundance of C12 P 2O O18 etc. Bypassing. Joel Emer. Computer Science & Artificial Intelligence Lab.. Massachusetts Institute of Technology. March 19, 2012. http://csg.csail.mit.edu/6.S078. L12-. 1. Six Stage Pipeline. March 19, 2012. Jeff Johnson. Feb 19, 2014. MS Proteomics in a Nutshell. Ionization. Delivering macromolecules to the MS. Ion Manipulation. Trapping and ejecting . analytes. of interest. Fragmentation. Breaking apart for more information. Outline. Tandem Mass Spectrometry. De Novo Peptide Sequencing. Spectrum Graph . Protein Identification via Database Search. Identifying Post Translationally Modified Peptides . Spectral Convolution . Tommi Eronen. Max-Planck-Institut für Kernphysik. Heidelberg, Germany. Outline. Penning trap mass spectrometry. Q. = . m. parent. – . m. daughter. . Masses for CKM unitarity tests.... Superallowed beta decay. PUMP How the magnetic sector mass spectrograph works: qvB = mv), B the magnetic field, m is the mass of the Classically, the speed of the ion, v, is related Courtesy www.lab-initio.com. Purpose of Mass Spectrometry. P. roduces.  . spectra of masses from . the molecules . in . a sample of . material, and fragments of the molecules.. U. sed . to determine . Know what information they can provide. Interpret simple mass spectra graphs. Know some uses of mass spectrometry. A mass spectrometer….. Draw the mass spectrometer. Describe the 5 step process. AS A CARTOON STORY BOARD!. Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. David Horn. Thermo Fisher Scientific . San Jose, CA. Outline. Challenges in the characterization of biopharmaceuticals. Introduction to the new generation of . Orbitrap. TM. . mass spectrometers. Confident intact antibody analysis using the Q . Fenyő. Readings for Today. http://fenyolab.org/pi2019. Ion Source. Mass Analyzer. Detector. mass/charge. intensity. Mass spectrometry . Mass Analyzer 1. Frag-mentation. Detector. Ion Source. Mass Analyzer 2. MS applications. Microarray analysis. CSE182. LC-MS Maps. time. m/z. I. Peptide 2. Peptide 1. x x x x. x x x x x x. x x x x. x x x x x x. time. m/z. Peptide 2 elution. A peptide/feature can be labeled with the triple (M,T,I):. &. Secondary. . ion. . mass. . spectrometry. (SIMS). L. Seda Mut 20970802 . Neslihan . Ötük. 20622809. Beytepe. Ankara. 12.04.2012. Outline. Historical Background of MS. . and. SIMS. What is M. SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con L7. Dicitionary. . matching. Pattern matching. Fa05. CSE 182. Dictionary Matching. Q: Given k words (s. i. has length l. i. ). , . and a database of size n, find all matches to these words in the database string..