PPT-CSE182 CSE182-L12 Mass Spectrometry

Peptide identification CSE182 General isotope computation Definition Let p ia be the abundance of the isotope with mass i Da above the least mass Ex P 0C abundance of C12 P 2O O18 etc. Keywords. :. Molecular . ion (M. +). Fragmentation. Starter:. Use mini wipe boards to brainstorm what you know about mass spectrometry. A: . Use . the molecular ion peak in an organic . molecule’s . PUMP How the magnetic sector mass spectrograph works: qvB = mv), B the magnetic field, m is the mass of the Classically, the speed of the ion, v, is related Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Fenyő. Readings for Today. http://fenyolab.org/pi2019. Ion Source. Mass Analyzer. Detector. mass/charge. intensity. Mass spectrometry . Mass Analyzer 1. Frag-mentation. Detector. Ion Source. Mass Analyzer 2. MS applications. Microarray analysis. CSE182. LC-MS Maps. time. m/z. I. Peptide 2. Peptide 1. x x x x. x x x x x x. x x x x. x x x x x x. time. m/z. Peptide 2 elution. A peptide/feature can be labeled with the triple (M,T,I):. Dynamic Programming. www.cse.ucsd.edu/classes/. fa09/. cse182. www.cse.ucsd.edu/~vbafna. FA08. CSE182. Notes. Assignment 1 is online, due next Tuesday.. Discussion section is optional. Use it as a resource.. L8. Protein Sequence Analysis . Patterns (regular expressions). Profiles. HMM. Gene Finding. October 09. CSE 182. QUIZ!. Question:. your ‘friend’ likes to gamble. . He tosses a coin: HEADS, he gives you a dollar. TAILS, you give him a dollar.. Lecture 1. 4/13/2017. Announcements. Homework 2.2 Additional Problems - Due. Quiz Today (after Announcements). 4/18 Lecture – will go back to rest of NMR (interpretation examples and instrumentation), then continue on Mass . &. Secondary. . ion. . mass. . spectrometry. (SIMS). L. Seda Mut 20970802 . Neslihan . Ötük. 20622809. Beytepe. Ankara. 12.04.2012. Outline. Historical Background of MS. . and. SIMS. What is M. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con L7. Dicitionary. . matching. Pattern matching. Fa05. CSE 182. Dictionary Matching. Q: Given k words (s. i. has length l. i. ). , . and a database of size n, find all matches to these words in the database string..