PPT-Protein Sequencing and Identification by Mass Spectrometry

Outline Tandem Mass Spectrometry De Novo Peptide Sequencing Spectrum Graph Protein Identification via Database Search Identifying Post Translationally Modified Peptides Spectral Convolution . chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. PUMP How the magnetic sector mass spectrograph works: qvB = mv), B the magnetic field, m is the mass of the Classically, the speed of the ion, v, is related Courtesy www.lab-initio.com. Purpose of Mass Spectrometry. P. roduces.  . spectra of masses from . the molecules . in . a sample of . material, and fragments of the molecules.. U. sed . to determine . Stefano . Lise. Bioinformatics & Statistical Genetics (BSG) Core. The . Wellcome. Trust Centre for Human Genetics (WTCHG), Oxford. Email: stefano@well.ox.ac.uk. Outline. Human genetic variation in health and disease. (very) large datasets. 5/23/17. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. David Horn. Thermo Fisher Scientific . San Jose, CA. Outline. Challenges in the characterization of biopharmaceuticals. Introduction to the new generation of . Orbitrap. TM. . mass spectrometers. Confident intact antibody analysis using the Q . Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con INTRODUCTION. PROTEIN SEQUENCING. WHY PROTEIN SEQUENCING MATTERS?. HISTORY. STRATEGY. SEQUENCING METHODS. APPLICATIONS. INTRODUCTION. PROTEIN. Proteins are polymers of amino acid. Protein's structure & function depends upon amino acid sequence.