PPT-Protein Sequencing and Identification by Mass Spectrometry

Outline Tandem Mass Spectrometry De Novo Peptide Sequencing Spectrum Graph Protein Identification via Database Search Identifying Post Translationally Modified Peptides Spectral Convolution . Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. Keywords. :. Molecular . ion (M. +). Fragmentation. Starter:. Use mini wipe boards to brainstorm what you know about mass spectrometry. A: . Use . the molecular ion peak in an organic . molecule’s . H.V.Florance@exeter.ac.uk. bs-mass-spec@exeter.ac.uk. http://biosciences.exeter.ac.uk/facilities/spectrometry/. The University of Exeter Science Strategy. – Systems Biology. HOW MASS SPECTROMETRY CAN IMPROVE YOUR RESEARCH. Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. David Horn. Thermo Fisher Scientific . San Jose, CA. Outline. Challenges in the characterization of biopharmaceuticals. Introduction to the new generation of . Orbitrap. TM. . mass spectrometers. Confident intact antibody analysis using the Q . Objectives of the presentation. To get an appreciation of the role of . physics . in . our day to day living. Acquaint ourselves to a novel and . widely used . research tool: Mass Spectrometer. To . introduce the basic physics of mass . Fenyő. Readings for Today. http://fenyolab.org/pi2019. Ion Source. Mass Analyzer. Detector. mass/charge. intensity. Mass spectrometry . Mass Analyzer 1. Frag-mentation. Detector. Ion Source. Mass Analyzer 2. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. Introduction to Biostatistics and Bioinformatics. Proteomics Informatics. This Lecture. Proteomics Informatics – Learning Objectives. Structure of . m. ass spectrometry data. Protein identification. chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con INTRODUCTION. PROTEIN SEQUENCING. WHY PROTEIN SEQUENCING MATTERS?. HISTORY. STRATEGY. SEQUENCING METHODS. APPLICATIONS. INTRODUCTION. PROTEIN. Proteins are polymers of amino acid. Protein's structure & function depends upon amino acid sequence.