PPT-PROTEIN SEQUENCING PRESENTATION OUTLINE

INTRODUCTION PROTEIN SEQUENCING WHY PROTEIN SEQUENCING MATTERS HISTORY STRATEGY SEQUENCING METHODS APPLICATIONS INTRODUCTION PROTEIN Proteins are polymers of amino acid Proteins structure amp function depends upon amino acid sequence. 2012 HHMI Summer Workshop for High School Science . Teachers. The Genomics of . S.cerevisiae. GOALS. Introduction to the Genomics of Yeast. Sequencing Technologies and how they are evolving. Introduction to Systems Biology and modern Yeast Genetics. Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. Stefano . Lise. Bioinformatics & Statistical Genetics (BSG) Core. The . Wellcome. Trust Centre for Human Genetics (WTCHG), Oxford. Email: stefano@well.ox.ac.uk. Outline. Human genetic variation in health and disease. Sequence:. Edman degradation. Mass spectrometry. Secondary structure:. Circular Dichroism. FTIR. Tertiary, quaternary structure:. NMR. X-ray crystallography. Protein sequencing approaches depend on what is known and what is the goal. (very) large datasets. 5/23/17. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes. RSVP. Lesson . #13: Tips for a Successful Senior Project PowerPoint. Know Your Audience. Your audience is: . Educators, business professionals and community members. Presentation First, PowerPoint Second. Steven J. Madore, Ph.D.. ICBR Associate Director for Science. UFHCC Associate Director for Core . Technologies. 2018 Pathology Research Infrastructure . Talk. April 11, . 2018. Talk Outline. The ICBR – . How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. Hardison. Genomics 2_1. 3/1/15. 1. Y Chromosome. 3 billion bp = 3 Gb. Chr1 247 Mb. Chr12 132 Mb. Chr22 50 Mb. A human . genome (. male). The . genome. is . all the DNA. in a cell.. All the DNA on all the chromosomes. mRNA. RNA-SEQ. TruSeq. . Stranded. . mRNA. RNA-SEQ. The first . step. in the . workflow. . involves. . purifying. the . poly-A. . containing. . mRNA. . molecules. . using. . poly-T. . oligo. DNA - the basics. Drew Berry – DNA animations. http://. www.youtube.com. /. watch?v. =. WFCvkkDSfIU&index. =4&list=PL9CBBEA5A85DBCDEF. Organisation of DNA. DNA is packed in Chromosomes . Karyotype: chromosome set of a species . What Molecule Codes For Genes?. What carries information between DNA to Proteins?. How Are Proteins Made? (Translation). How Do Individuals of a Species Differ?. Why Bioinformatics?. Most materials revised from http://www.bioalgorithms.info . Update and rationale. Low-pass sequencing (Goncalo). Analysis . Disease projects (Sardinia, T2D). A public database of. . SNPs. . and detectable . CNVs. with allele frequency. >1. % in each of multiple human population samples.