PPT-Mass Spectrometry

Courtesy wwwlabinitiocom Purpose of Mass Spectrometry P roduces   spectra of masses from the molecules in a sample of material and fragments of the molecules U sed to determine . chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. Jeff Johnson. Feb 19, 2014. MS Proteomics in a Nutshell. Ionization. Delivering macromolecules to the MS. Ion Manipulation. Trapping and ejecting . analytes. of interest. Fragmentation. Breaking apart for more information. Outline. Tandem Mass Spectrometry. De Novo Peptide Sequencing. Spectrum Graph . Protein Identification via Database Search. Identifying Post Translationally Modified Peptides . Spectral Convolution . Tommi Eronen. Max-Planck-Institut für Kernphysik. Heidelberg, Germany. Outline. Penning trap mass spectrometry. Q. = . m. parent. – . m. daughter. . Masses for CKM unitarity tests.... Superallowed beta decay. Know what information they can provide. Interpret simple mass spectra graphs. Know some uses of mass spectrometry. A mass spectrometer….. Draw the mass spectrometer. Describe the 5 step process. AS A CARTOON STORY BOARD!. Jonathan A. Karty, Ph.D.. jkarty@indiana.edu. http://msf.chem.indiana.edu . Why Mass Spectrometry. Information is composition-specific. Very selective analytical technique. Most other . spectroscopies. David Horn. Thermo Fisher Scientific . San Jose, CA. Outline. Challenges in the characterization of biopharmaceuticals. Introduction to the new generation of . Orbitrap. TM. . mass spectrometers. Confident intact antibody analysis using the Q . Peptide identification. CSE182. General isotope computation. Definition:. Let p. i,a. be the abundance of the isotope with mass i Da above the least mass. Ex: P. 0,C. : abundance of C-12, P. 2,O. : O-18 etc.. April 15. Bafna. Peptide MS. Instrument software usually detects peaks, and computes features (peak, area, m/z…). April 15. Bafna. m/z. Nobel Citation 2002. April 15. Bafna. Nobel Citation, 2002. April 15. &. Secondary. . ion. . mass. . spectrometry. (SIMS). L. Seda Mut 20970802 . Neslihan . Ötük. 20622809. Beytepe. Ankara. 12.04.2012. Outline. Historical Background of MS. . and. SIMS. What is M. and Andrew Gioe. Computation of Molecular Mass. Mass Spectrum is a plot of intensity as a function of mass-charge ratio, m/z. . Mass-charge ratio can be determined through accelerating an ion.. Conti. chromatographyand.  mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation, and identification of unknown samples. GC-MS can also be used in airport security to detect substances in luggage or on human beings. Additionally, it can identify trace elements in materials that were previously thought to have disintegrated beyond identification.. Mass Spectrometry deals with the examination of characteristics fragments and arising from the breakdown of organic molecules. The mass spectrum is the plot of relative intensity of the ions agains SOP template 1 Revised: 06 /2019 MTD Standard Operating Procedure (SOP) This Standard Operating Procedure (SOP) describes basic chemical safety information for Mass Spectrometry (MS) . Prior to con