PPT-Chapter 10 Control of Gene Expression

101 Between You and Eternity Cancer is a multistep process in which cells grow and divide abnormally disrupting physical and metabolic functions More than 200000 new cases of breast cancer are diagnosed in the US each year about 5700 in women and men . And 57375en 57375ere Were None meets the standard for Range of Reading and Level of Text Complexity for grade 8 Its structure pacing and universal appeal make it an appropriate reading choice for reluctant readers 57375e book also o57373ers students Bing Zhang. Department of Biomedical Informatics. Vanderbilt University. bing.zhang@vanderbilt.edu. Overall workflow of gene expression studies. Microarray. Biological question. Experimental . design. What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. Advances in Gene Expression Science . Allow Scientists to Compare & Contrast . Expression Patterns of two Biological States. Biological State #1. Biological State #2 State. Comparison of Genetic Profiles of Adipose Tissue in Identical Twins . Teachable Unit. Gene Expression Team. NANSI 2013, University of Minnesota. Participants: Michael Burns, Lucy He, David Kirkpatrick, Bridget Lear, Tamar Resnick, Turk Rhen. Facilitators: Judy Ridgway, Sue Wick. University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. Chapter . 16. Genes and Development. Proteins can determine the DNA sequence by binding the major groove of DNA.. Proteins binding the minor groove cannot determine the exact sequence of bases.. Prokaryotic gene regulation. Anablep. . anablep. . with its “4 eyes”. Upper half of lids look aerially while the lower half looks into the water.. Cells of the two parts of the eye exhibit differential gene expression. Individual bacteria respond to environmental change by regulating their gene expression. How do we regulate the expression of our genes? . Involved in gene expression. DNA regulatory sequences. Regulatory genes. Small regulatory proteins (. RNAs. ). Regulatory sequences. Stretches of DNA that interact with regulatory proteins to control transcription.. analysis - outliers. V2: batch effects. V3: data imputation. What . is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g  which is designed to allow . expression.  (. transcription.  and . translation. ) of the inserted section of . DNA. . The . vector.  carries a . promoter.  (normally inducible) on one side of the . Pearley Chinta and Juliet V. Spencer . Abstract. Methods. Results. Conclusions. HCMV is a widespread pathogen in the general population and can cause severe disease in immune-compromised hosts. HCMV manipulates immune responses in several ways, one of which includes encoding genes with homology to host chemokine receptors. HCMV US27 encodes a chemokine-like receptor that stimulates host gene expression. While, no chemokine ligand has been identified for US27, it is constitutively active. US27 stimulates the gene expression of antioxidant response element (ARE) regulated genes by activation of the transcription factor nuclear respiratory factor 1 (NRF-1). The goal of this project is to identify specific host and viral genes that are regulated by US27. Increased expression of antioxidant genes is likely to benefit virus infection and enable more progeny virus to be produced. Thus, a better understanding of the US27 function has the potential to lead to the development of novel antiviral therapies necessary to treat HCMV infection. . What is measured by microarrays?. Microarray normalization. Differential gene expression (DE) analysis based on microarray data. Detection of outliers. RNAseq. data. DE analysis based on . RNAseq. data.