PPT-Drug Repurposing for Various Courses of COVID-19 Based on Single-cell RNA Sequencing Data

Kai Guo 1 Zhihan Wang 12 Pan Gao 1 Qinqin Pu 1 Min Wu 1 Changlong Li 2 and Junguk Hur 1 1 Department of Biomedical Sciences University of North Dakota School of Medicine and Health Sciences Grand Forks ND 58202 USA. DNA pol error rate . ~. 10. -9. per base . copied. How many errors in a . “typical” . somatic cell?. Most errors don’t have detectable effects. But some errors do: . . oncogenes. N. . – dominant. INTRODUCTION . The most commonly used technology until a few years ago – BAC. WHOLE GENOME SEQUENCING. ADVANTAGES OF WGS. Utility of next – gen sequence reads . The next-generation platforms are effecting a complete paradigm shift, not only in the organization of large-scale data production, but also in the downstream bioinformatics, IT, and LIMS support required for high data utility and correct interpretation.. A I . Bhat. Indian Institute of Spices Research. Calicut. DNA sequencing. Chain termination method . (. Sangers. . et a. l., 1977): In this method, the sequence of a single stranded DNA molecule is determined by enzymatic synthesis of complementary polynucleotide chains, these chains terminating at specific nucleotide positions.. (very) large datasets. 5/24/18. Goals for the course. Understand how next-generation sequencing technologies are used in biomedical research. Learn how to use publicly available databases/websites to find specific information about genes.  . Nearly . all modern DNA sequencing procedures require a concentrated amount of . single-stranded . DNA known as a . template. . A template is simply a piece of DNA of . sufficient . length and quality to allow for its sequencing (most sequencing methods . Hailun Wang. , Pak Sham, Tiejun Tong and . Herbert Pang. Rocky 2019. December 7. th. (Saturday). 2. W. hy . scRNA. -Seq data. Propose a pathway-based analytic framework using Random Forests. Identify discriminative functional pathways related to cellular heterogeneity . TexPoint fonts used in EMF: . A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. A. Lecture 1. Instructor:. David Tse. dntse@stanford.edu. The Genome. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. He. 2019-10-31. The Core of Biology Is All About One Cell. Forward Approaching. The Nobel Prize in Physiology or Medicine 2004. Dr. Richard Axel . Dr. Linda Buck. The odorant receptors are likely to belong to the superfamily of receptor proteins that transduce intracellular signals by coupling to GTP-binding proteins.. Although . genome profiling studies have demonstrated heterogeneity in subclonal architecture that may ultimately lead to relapse, a gene-expression based prediction program that can identify, distinguish and quantify drug response in subpopulations within a bulk population of myeloma cells is lacking. . Peptidase . IV (DPP4) . Inhibitors . as . Potential Agents . to treat SARS-CoV-2 (2019-nCov) . Infection. DPP4 activity is increased in patients with type-2 diabetes and the increased risk of type-2 diabetic patients to SARS-CoV-2 infection, which suggests that DPP4 class of drugs have the potential to be used as novel therapy. Our molecular modeling studies suggest that. for A. nalysis of Single . C. ell RNA-. Seq. Data . . Ion . M. ă. ndoiu. Computer . Science & Engineering Department. University of . Connecticut. ion@engr.uconn.edu. Outline. Intro to . scRNA-Seq. Aaron D. Schroeder, Ph.D.. Senior Data Research Scientist. Social & Decision Analytics Lab. Biocomplexity Institute of Virginia Tech. Every Repurposing Is a New “Investigation”. Locating the Data Repurposing Discussion. Virupaksha. A. Bastikar. 1. ; . Pramodkumar. Gupta. 2. ; Alpana V. Bastikar. 3. and Santosh S. Chhajed. 4. 1. Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra;. 2. School of Biotechnology and Bioinformatics, D. Y. . Seq. Analysis Pipeline. Marmar. Moussa. Ion . Mandoiu. Computer . Science & Engineering Department. University of . Connecticut. Outline. Motivation and challenges. Locality sensitive imputation.