PPT-Create an Atlas of Gene Expression during Craniofacial Development

Potter Brunskill Trainor Aronow Combination Laser CaptureTransgenicGFP and Microarray Nex Gen Compared three technologies Nugen One Direct RiboSpia technology μMACS Super Amp Kit from . Inducible gene expression. kinetics of . β-galactosidase. enzyme induction. Add inducer. start transcription = mRNA accumulation. mRNA translation = protein accumulation. Remove inducer. Stop. transcription (. The problem to be solved (an example). Hauf, J., Zimmermann, F.K., M. ü. ller, S., 2000. Simultaneous genomic over expression of seven glycolytic enzymes in the yeast Saccharomyces cerevisiae. Ezyme. Microbiol. Technol.. Gene in . Arabidopsis. Maximilian A. Busch,* Kirsten Bomblies,* Detlef Weigel†. Presentation by . Steven Wang and Clemens Langemeyer. Content:. Introduction. Objective(s) / Hypothesis . What do we know?. zebrafish. Adam Contreras. Postlethwait. Lab. microRNA. (. miR. ). Non-coding RNA 18-22 nucleotides long. Represses mRNA translation. http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/microrna-studies/tech-notes/micrornas-processing.html. What does the operon model attempt to explain?. the coordinated control of gene expression . in bacteria. bacterial resistance to antibiotics. how genes move between homologous regions of DNA. the mechanism of viral attachment to a host cell. University of Notre Dame. Kristin Hager, Associate Teaching Professor, Biological Sciences. Joseph O’Tousa, Professor, Biological Sciences. WH Freeman. Marc Mazzoni, Senior Editor, Life Sciences. Elaine Palucki, Editor, Adjunct Assistant Professor Biology, Brooklyn College. The cerebellum is the region of the brain that coordinates motor control, and many human neural disorders impact the cerebellum.. Several spontaneous mouse mutants with motor defects have been found, including:. Gene Expression. Gene Expression. Transcription. Splicing. Polyadenylation. mRNA Stability. Translation. Protein Stability. Controls on Protein Levels. Transcription Control - Prokaryotic Promoter. Transcription Control - Prokaryotic Promoter. Drosophila. Embryos using . lacZ. Transgenes. June 18. th. ABLE 2014. University of Oregon, Eugene. Cathy Silver Key. Julie Gates. Jessica Sawyer. Kirsten . Guss. Acknowledgements. Funding from . Roberta . ArrayExpress. and Gene Expression Atlas:. Amy Tang, PhD. ArrayExpress. Production Team. Functional Genomics Group. EMBL-EBI. What’s covered this morning?. What do we mean by “functional genomics data”? Why do we need databases for them?. We performed a genome-wide analysis of gene expressionto identify germline- and sex-regulated genes.Using mutants that cause defects in germ cell proliferationor gametogenesis, we identiÞed sets of g Coordinated growth and differentiation of externalgenitalia generates a proximodistally elongated structuresuitable for copulation and efficient fertilization. Thedifferentiation of external genitalia A Big Thanks. Prof. Jason . Bohland. Quantitative Neuroscience Laboratory. Boston University. The Process. Construction and representation of the Anatomic Gene Expression Atlas (AGEA). . Allen Reference Atlas. Developing Ovule,. . Embryo and Endosperm. The 2. nd. . International . Conference on Transcriptomics. Jianbo. Wang. Wuhan University, China. Background. Materials and methods. Gene expression. .