PPT-The Yeast ORFan /GUF Gene

Project Discovery of yeast gene function in undergraduate courses wwwyeastorphanprojectcom Jill Keeney Juniata College PA tammy tobin Susquehanna university PA Who We Are Arizona State University. Michelle . Attner. July 24, 2012. What is budding yeast, . S. . cerevisiae. ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter). budding yeast as a model for eukaryotic biology. Stacia R. Engel. Stanford University. Challenge to Biocurators. Promote ways in which [. your_organism. ] . and . r. esearch on . [. your_organism. ] . Biochemistry and genetics provide a powerful combination for analyzing cell function. Yeast geneticists frequently invoke:. KEGG (Kyoto Encyclopedia of Genes and Genomes – . S. cerevisiae. metabolic map. assay for . RNA-protein . interactions. Dana M. . Schneider. Loren . Williams lab. Outline. The . Ribosome. Yeast . three hybrid assay for RNA-protein . interactions. Construct vectors. PCR amplify insert. YSC1021 Yeast: Yeast Knock Out StrainYSC4300Yeast Heterozygous (plates 281-282)YSC1053Yeast MATa CollectionYSC4343Yeast Heterozygous Plate 282YSC4298Yeast MATa (plates 72-73)YSC4508Yeast Heterozygous ORFans. to . “GO”. Genomic sequence of model eukaryote . Saccharomyces cerevisiae . completed in 1996. (12.1 Mb). Despite 16 years of intense research, function of nearly 30% of putative open reading frames (ORFs) remain unknown.. Interactions. June 6, 2017. Why PPI?. Protein-protein interactions determine outcome of most cellular processes. Proteins which are close homologues often interact in the same way. Protein-protein interactions place evolutionary constraints on protein sequence and structural divergence. June 12, 2018. Why PPI?. Protein-protein interactions determine outcome of most cellular processes. Proteins which are close homologues often interact in the same way. Protein-protein interactions place evolutionary constraints on protein sequence and structural divergence. Dr. Brian Rymond (Instructor). &. Christen . Wanstrath. (TA). Make and break DNA (and RNA) in a variety of ways and test the consequences in the host organism (. E. coli, S. cerevisiae, C. elegans. Raghav. . Ramachandran. Ambhi Ganesan. September 9,2008. A model for life science research. Test out various hypotheses on a smaller scale.. Saves time and money.. Practically impossible to carry out certain research directly on intended targets.. Caenorhabditis. . elegans. Insuk. Lee1,4, Ben Lehner2,3,4, . Catriona. Crombie2, Wendy Wong2, Andrew G Fraser2 & Edward M Marcotte1. Abstract. The fundamental aim of genetics is to understand how an organism's . [22]. The pattern of blocks was assessed to see whether theirarrangement in the ancestral genome prior to duplicationarrangement in the ancestral genome prior to duplicationequally parsimonious series 2040G1G1G2G26080100120140160 GeneChip Specifications Feature size Standard format Oligo length25mers Probe pairs/gene~ Detection SensitivityPoly A controlsdap, lys, phe, thr, trpYeast controlsActin, Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .