PPT-Combination of fluorescence FRET microscopy and optical tweezers for mechanical studies

Camille Dubois Biophotonics group Laboratoire Charles Fabry Institut dOptique Under the supervision of Nathalie Westbrook SFP 7 juillet 2023 Ludivine Houel Renault Marie . BIF Microscopy Workshop. March 25. th. , 2015. Director: Professor Thomas V. O’Halloran. Managing Director: Keith MacRenaris, . Ph.D. History and Mission of CLP and QBIC. “The . Chemistry of Life Processes Institute acts as an umbrella for a variety of centers, facilitates collaborations and helps bridge different cultures. By lowering the barriers to scientific discovery, the Institute hopes, for example, to design new drugs for the treatment of cancer and neurodegenerative diseases as well as develop improved techniques for diagnosing diseases earlier.” . Microlens. Array. 18 October, . FiO. 2011. Antony Orth and Kenneth . Crozier. High Throughput Microscopy. 1. http://www.olympus.co.uk/microscopy/22_scan_R.htm#. High throughput fluorescence imaging by scanning sample under . Ozkans. ’. laboratory in UC-Riverside to perform defect analysis and surface metrology of large-area CVD-graphene sheets. This method utilizes the quenching of fluorescence from dye molecules by graphene via resonant energy transfer to increase the visibility of graphene on a glass substrate. A large-area fluorescence montage image of the dyed graphene sample is collected and processed to identify the graphene and indicate the graphene layer thickness throughout the entire graphene sheet. Furthermore, chemically functionalized (doped with fluorine) parts of graphene film is visualized using the same technique. The emission of the dye is quenched to a different extent by fluorinated and pristine graphene, which provides the fluorescence-imaging contrast essential for this method. The regions with pristine graphene appear darker on the fluorescence images than the regions with fluorinated graphene, enabling large-scale mapping of the functionalized regions in CVD grown graphene sheets. Complex circular patterned regions of doped and pristine graphene regions are resolved with great accuracy. This method is posed for widespread adoption by graphene manufacturers as a basis for facile and high throughput metrology of large scale graphene sheets. Finally, this work featured as cover articles in . Authorized Distributor & Only Service Center in India. INDE ENTERPRISES. http://www.smarttweezers.co.in. We sell and repair . Smart Tweezers. INDE Enterprises is the first authorized distributors for Smart Tweezers in India since 2005.. Mentor: Prof. Dr Igor Poberaj. University of Ljubljana. Faculty of Mathematics and Physics. Microrheology with optical tweezers. Ljubljana, December 4th, 2012. Introduction. Microrheology. Optical tweezers. Alexandra Kent & Allyson Brome. University of Utah. Non Covalent Interactions. Wiki Page. : . http. ://en.wikipedia.org/wiki/Non-. covalent_interactions. . Other References: . Anslyn. , E. V., Dougherty, D. A. (2006). Modern Physical Organic Chemistry. Sausalito, CA. University Science.. Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. and Kavantzas N., "Computer vision algorithms in DNA ploidy image analysis", Imaging, Manipulation and Analysis of Biomolecules, Cells and Tissues IV, Proc. SPIE, 6088:60880O (2006) and Loukas S., "A Replica of van 1670 Moody Use the information in this tutorial to supplement the visuals in lab and the Chapters 1 8 and 9 in your lab manual Replica of Culpepper tripod microscope built c 1725 by Co Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. are held together at their centromeres binds specific proteins, which in turn make up a disk-like structure called the kinetochore. The kinetochore is an attachment site for spindle fibers, which pla Reif. Outline. Fluorescent resonance energy transfer (FRET). Fluorescent Labels. Fluorophores. , quantum dots. Single FRET. Molecular Beacon. DNA Tweezers. Multiple FRET. Homo-FRET. Hetero-FRET. Optically-induced molecules. A . fluorescence microscope.  is an optical microscope that uses fluorescence and phosphorescence instead of, or in addition to, reflection and absorption to . study . organic . or inorganic substances.