Lecture1 Dr Shaimaa Munther Physiology The science that is concerned with the function of the living organism and its parts and of the physical and chemical processes involved General Principles The Body as an Organized ID: 1031842
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1. PhysiologyThe Body as an Organized "Solution”Lecture:1Dr. Shaimaa Munther
2. PhysiologyThe science that is concerned with the function of the living organism and its parts, and of the physical and chemical processes involved.
3. General Principles : The Body as an Organized "Solution”The cells that make up the bodies of all but the simplest multicellular animals, exist in an "internal sea" of extracellular fluid (ECF) enclosed within the integument of the animal. The ECF is more dilute than present-day seawater, but its composition closely resembles that of the primordial oceans.From this fluid, the cells take up O2 and nutrients; into it, they discharge metabolic waste products
4. Body Fluids Water, (the primary body fluid) is the most important nutrient for life.≈ 40-75% of human body weight is water. Loss of 10% body fluid = 8% weight loss SERIOUSLoss of 20% body fluid = 15% weight loss FATALFluid gained each day should = fluid lost each day (2 -3L/day average)
5. Functions of Body FluidMedium for transportNeeded for cellular metabolismSolvent for electrolytes and other constituentsHelps maintain body temperatureHelps digestion and eliminationActs as a lubricant
6. Body Fluid CompartmentsIn lean adults, body fluids constitute 55% of female and 60% of male total body mass these divided into:Intracellular fluid (ICF) inside cells About 2/3 of body fluid Extracellular fluid (ECF) outside cells divided into :Interstitial fluid ( fluid between cell) is 80% of ECFIntravascular fluid (Plasma in blood )is 20% of ECF
7. Body Fluid Compartments
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9. Fluid balanceNote: Insensible loss = 1000 cc/day
10. During transfer on nutrients (chemicals) an average 24 liters of fluid moves out of plasma each day; 85% is reabsorbed & the remainder (4L) is the lymph fluid that accumulatesAt the capillary level have 2 separate forces at work:Hydrostatic pressure --- pushes fluid out of vesselsColloid osmotic pressure --- pushes fluid into vessels
11. In addition to water, the body contains solutes; substances that separate in solutionSolution = Solute + SolventSolution = the fluid compartment (ICF or ECF)Solvent = waterSolutes:ElectrolytesCharged inorganic ionsThey dissolve in waterThey can conduct an electric currentMajor cations:ECF = sodium ICF = potassiumMajor anions:ECF = chloride, bicarbonate ICF = proteins neg chargeNon-electrolytesPolar = those that are water soluble (sugars, proteins)Non-polar = those that are water insoluble (lipids)Composition of body fluids
12. Water, Electrolytes, & Acid/Base
13. Life is evolved in water and is still absolutely dependent on it. WaterWater (molecular formula H2O) is an essential part of all living organisms, making up 70% or more of the weight of most organisms. It consists of an oxygen atom connected to two hydrogen atoms by polar covalent bonds. It is considered to be the universal solvent for many reasons including its structural, chemical and physical properties. These properties result in the many unique characteristics of water.
14. Properties of waterVery polarOxygen is highly electronegativeH-bond donor and acceptorHigh boiling point , melting point, heat of vaporization, surface tension
15. Polarity of WaterIn a water molecule two hydrogen atoms form single polar covalent bonds with an oxygen atom. Gives water more structure than other liquidsBecause oxygen is more electronegative, the region around oxygen has a partial negative charge.The region near the two hydrogen atoms has a partial positive charge.A water molecule is a polar molecule with opposite ends of the molecule with opposite charges.
16. Water dissolves polar compoundssolvation shellorhydration shell
17. Hydrogen Bonding of WaterHydrogen bonds hold water molecules together.Each water molecule can form a maximum of 4 hydrogen bonds.The hydrogen bonds joining water molecules are weak, about 1/20th as strong as covalent bonds.They form, break, and reform with great frequency
18. Ionization of Water
19. H20 + H20 H3O+ + OH- [H+] [OH-] Keq = [H2O] H20 H+ + OH-Keq=1.8 X 10-16M[H2O] = 55.5 MIf [H+] = [OH-] then [H+] = 1.0 X 10-7 Ionization of Water[H+] [OH-] = (1.8 X 10-16)(55.5 ) [H2O] Keq = [H+] [OH-]Kw =[H+] [OH-] = 1.0 X 10-14
20. ElectrolytesElectrolytes are ions capable of carrying an electric charge. They are classified as anions or cations based on the type of charge they carry. CATIONS (+) ANIONS (-) (e.g, NaCl) are molecules that dissociate in water to their cation (Na+) and anion (Cl–) equivalents. Because of the net charge on water molecules, these electrolytes tend not to re associate in water. There are many important electrolytes in physiology, notably Na+, K+, Ca2+, Mg2+, Cl–, and HCO3–. It is important to note that electrolytes and other charged compounds (eg, proteins) are unevenly distributed in the body fluids .These separations play an important role in physiology
21. ElectrolytesCationsPositively chargedSodium Na+Potassium K+Calcium Ca++Magnesium Mg++AnionsNegatively chargedChloride Cl-Phosphate PO4-Bicarbonate HCO3-
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24. Electrolyte Functions Electrolytes are an essential component in numerous processes, including:Volume and osmotic regulation (Na, Cl & K)Myocardial rhythm and contractility (K, Mg & Ca2). Cofactors in enzyme activation (e.g., Mg, Ca). Regulation of adenosine triphosphatae, (ATPase) ion pumps (Mg)Acid-base balance (HCO3, K & Cl)Blood coagulation (Ca, Mg).Neuromuscular excitability (K, Ca, Mg). The production and use of ATP from glucose (Mg,PO4).
25. pH & The body Buffering SystemBuffers are compounds that resist changes in pH upon the addition of limited amounts of acids or bases.Buffer systems are usually composed of a weak acid or base and its conjugate salt. The components act in such a way that addition of an acid or base results in the formulation of a salt causing only a small change in pH.The maintenance of a stable hydrogen ion concentration ([H+]) in body fluids is essential to life. The pH of a solution is defined as the logarithm to the base 10 of the reciprocal of the H+ concentration ([H+]), ie, the negative logarithm of the [H+]. The pH of water at 25 °C, in which H+ and OH–ions are present in equal numbers, is 7.0
26. pH ScaleDevised by Sorenson (1902) [H+] can range from 1M and 1 X 10-14M Using a log scale simplifies notation pH = -log [H+] Range is from 0 - 14Neutral pH = 7.0If [H+] is high, the solution is acidic; pH < 7If [H+] is low, the solution is basic or alkaline ; pH > 7
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28. Weak Acids and Bases Equilibrium Molecules that act as H + donors in solution are considered acids, while those that tend to remove H+ from solutions are considered bases. Strong acids (eg, HCl) or bases (eg, NaOH) dissociate completely in water and thus can most change the [H+] in solution e. g. (HCl , NaOH). Weak acids / bases – disassociate only partially e. g. (Acetic acid, Carbonic acid)
29. Acid/conjugate base pairs HA + H2O A- + H3O+ HA A- + H+ HA = acid ( donates H+) (Bronstad Acid) A- = Conjugate base (accepts H+) (Bronstad Base) Ka & pKa value describe tendency to loose H+ large Ka = stronger acid small Ka = weaker acidKa = [H+][A-] [HA] pKa = - log Ka
30. Henderson-Hassel bach Equation2) [H+] = Ka [HA] [A-]3) - log [H+] = - log Ka - log [HA] [A-]4) - log [H+] = - log Ka + log [A-] [HA]5) pH = pKa + log [A-] [HA]HA = weak acidA- = Conjugate base* H-H equation describes the relationship between pH, pKa and buffer concentrationHA A- + H+1) Ka = [H+][A-] [HA]
31. Similarly, the dissociation constant, or Kb value, of a weak base is given by the equation:and the buffer equation for weak bases, which is derived from this relationship, may be expressed as:
32. The Body Buffering System Enzymatic activity and protein structure are frequently sensitive to pH; in any given body or cellular compartment, pH is maintained to allow for maximal enzyme/protein efficiency.Body pH is stabilized by the buffering capacity of the body fluids. A buffer is a substance that has the ability to bind or release H+ in solution, thus keeping the pH of the solution relatively constant despite the addition of considerable quantities of acid or base. Of course there are a number of buffers at work in biological fluids at any given time. All buffer pairs in a homogenous solution are in equilibrium with the same [H+]; a great deal about all of the biological buffers in that system.
33. Normal acid-base balance is achieved through number of buffer systems :1- Bicarbonate-carbonic acid buffer system2- Phosphate buffer system 3- Protein buffer systems ( Amino acids & Hb ) The Body Buffering System
34. Bicarbonate/Carbonic Acid Buffer Carbonic acidBicarbonateExcreted in urineExcreted by lungsCarbon dioxide Bicarbonate /Carbonic Acid Buffer is the most important buffering system in the body . Carbonic acid is a weak acid , that only partly dissociated into H and bicarbonate. A unique feature of bicarbonate is the linkage between its buffering ability and the ability of lung to remove carbon dioxide from the body.
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37. Maintenance of normal protein and electrolyte concentrations controls water distribution in the body compartmentsWater passes freely across cell membranesIons and proteins cannot freely diffuse across most cell membranesWater moves passively in response to changes in solute concentrationAll monitors to homeostatic balance occur in ECF and not ICFFluid shifts occur between ICF & ECF in response to changes only in ECFKey to equilibrium is maintaining solute concentration (osmolality ) Note: water follows salt ,thus, regulation of fluid balance & electrolyte balance are intertwined Basic concepts relating to regulation of fluid & electrolytes
38. OsmolalityOsmolality is physical property of a solution that is based on the concentration of solute in term of miliosmoles per kilogram of solvent (w/w) & is the ability of that solution to cause osmosis between ICF & ECF.A low serum osmolality means a higher than usual amount of water in relation to the amount of particles dissolved in it ( over hydration or edema )An increased serum osmolality indicate deficient fluid volume.Measurment of serum osmolality gives information about the hydration status within the cells because of the osmotic equilibrium that is constantly being maintained on either side of the cell membrane .If change to hypertonic ECF, then water into ECFIf change to hypotonic ECF, then water into ICF
39. OsmosisMovement of water between two compartments by a membrane permeable to water but not to soluteWater moves from low solute to high solute concentration , requires no energy
40. osmolalityDetermining the osmolality is important because it indicates the water& electrolytes balance of the body. To determine the state of body water balance, one can measure plasma osmolality. Osmolality normal valuesPlasma : 275-295 mOsm/Kg24 hour urine : 300-900 mOsm/Kgurine/serum ratio : 1.0-3.0A solution that has HIGH osmolality is one that is > plasma osmolality = HYPERTONIC solutionA solution that has LOW osmolality is one that is < plasma osmolality = HYPOTONIC solutionA solution that has equal osmolality as plasma = ISOTONIC solution
41. Regulation of OsmolalityOsmolality of ECF is controlled by :Hypothalams by osmoreceptor via :Thirst MechanismRegulation of vasopressin ( antidiuretic hormone ADH) The Kidney via the control of Na concentration & regulation of blood volume (Renin-angiotensin-aldosterone system)
42. To maintain a normal plasma osmolality ( 275– 295 mOsm/kg of plasma H2O), osmoreceptors in the hypothalamus respond quickly to small changes in osmolality. A 1%–2% increase in osmolality causes a fourfold increase in the circulating concentration of ADH, and a 1%–2% decrease in osmolality shuts off ADH production. ADH acts by increasing the reabsorption of water in the collecting tubules. glucose (mg/dl) BUN (mg/ dl)Osmolality = 2Na + + 18 2.8 BUN = Blood urea nitrogen
43. TonicityNormally, cells neither shrink or swell because intracellular and interstitial fluids have the same osmolalityIncreasing osmolality of interstitial fluid draws water out of cells and cells shrinkDecreasing osmolality of interstitial fluid causes cells to swell
44. TonicityIsotonicHypertonic Hypotonic
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