PPT-Advanced Optical Microscopy

lecture 4 February 2013 Kai Wicker Exam written exam 26 February 2013 exact time and place will be announced by email Today The quantum world in microscopy 1 Photon antibunching 2 . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Lecture . 7: Confocal Microscopy. Optical Sectioning: adding the third dimension. Wide-field . Imaging. Point . Spread Function. Deconvolution. Features Essential for Diagnosis. Ulysses J. Balis, MD. Director, Division of Pathology Informatics. Associate Professor of Pathology. Department of Pathology. University of Michigan. ulysses@med.umich.edu. Adrian Pieri, Tom Nowak and Olivia Hughes. Introduction. The project that we undertook was about the exploration and analysis of various methods such as laser diffraction, optical microscopy and Atomic Force Microscopy in order to measure the width of a single strand of human hair using concepts of nanotechnology and microscopy.. Lecture 13:. Super-resolution microscopy: Part I. Lecture 13: . Fluorescent labeling, multi-. sprectral. imaging and FRET. Review of previous lecture. FRET. FLIM. Super resolution . microscopy. NSOM. Lecture 18:. High speed microscopy, Part 2. High speed microscopy, Part . 2: Spatial . light modulator microscope and other 3D sensors. Making laser scanning confocal microscopes faster. Resonant scanner confocal. Networks. (S. ONET). Advanced Computer Networks . SONET Outline. Brief History. SONET Overview. SONET Rates. SONET Ring Architecture. Add/Drop Multiplexor (ADM). Section, Line and Path. Virtual Tributaries. ( Fluorescence lifetime imaging). —. Molecular interraction (FRET). —. intracellular pH. etc. etc etc. . Pulsed IR-laser. ( Multiphoton exitation). —. Intracellular Tracking. —. Uncaging & Photostimulation . Lecture 16:. Super-resolution microscopy: Part . 2. Lecture 16: Super-resolution microscopy and TIRFM. Single molecule imaging. Total internal reflection fluorescence microscopy (TIRFM). Super-resolution techniques . Lecture . 07: . Confocal Microscopy. Adding the Third Dimension. Andres Collazo, Director Biological Imaging Facility. Wan-. Rong. (Sandy) Wong, Graduate Student, TA. Lecture . 7: Confocal Microscopy. 1Many different types of light microscopesare available each with their own strengths and limitationsA main tenant of good microscopy is to select the right tool microscope for the job at handPlease c POPULAR SCIENCE BACKGROUNDHow the optical microscope became a nanoscopeEric Betzig Stefan W Hell and William E Moerner are awarded the Nobel Prize in Chemistry 2014 for having bypassed a presumed scie Outline. Introduction. Optical Microscopy. Types of Optical Microscopes. Confocal Microscopy. Laser Scanning Confocal Microscopy. Examples. Optical Microscopy. Optical microscopy is the oldest type of microscope and uses visible light and a system of lenses to create an image of the sample. . Electron microscopy (EM) . is an electron beam which is focused into a small probe across the surface of a specimen . . The first electromagnetic lens was developed in 1926 by . Hans Busch. .. . Theoretically a microscope is an array of two lenses Need of M icroscopy Living organisms, cells and their organelles which are too small to be seen with the human eye, can only be seen with the aid