PPT-Module introduction, genomes and sequencing projects

Dr Vladimir Teif BS222 Genome Science Lecture 1 Welcome to BS222 Genome Science Meet your lecturers Vladimir Teif module supervisor Leonard Schalkwyk Patrick VargaWeisz Jordi Paps. From Swab to Publication. Madison I. Dunitz. 1. , David A. Coil. 1. , Jenna M. Lang. 1. , Guillaume Jospin. 1. , Aaron E. Darling. 2. , Jonathan A. Eisen. 1. UC Davis Genome Center. 1. University of California, Davis; . Last lecture summary. recombinant DNA technology. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. Designing. . your. . Sequencing. Run. https://genohub.com/next-generation-sequencing-guide/. Sequencing strategy. Genome size and genome complexity?!. . related organism, PFGE, flow . cytometry. Noncoding. Stefano . Lise. Bioinformatics & Statistical Genetics (BSG) Core. The . Wellcome. Trust Centre for Human Genetics (WTCHG), Oxford. Email: stefano@well.ox.ac.uk. Outline. Human genetic variation in health and disease. Production Genomics Facility. “. User facility. providing high-throughput DNA sequencing & analysis in support of DOE missions in alternative . energy. , . carbon cycling . & . bioremediation. Next Generation Sequencing. Hanlee Ji, M.D. . ã . Stanford University. Overview. Principles of next generation . DNA sequencing. Analysis of genetic variation and research applications. DNA. Double helix. Bases. Sequence. Gene . Key words. Genome . Genome sequencing. Species . Human Genome Project. DNA. is found in the nucleus of a cell. It is a long molecule that carries a set of codes to make you unique.. 6.1 Introduction to Genomics. 6.2 Sequencing Genomes. 6.3 Bioinformatics and Annotating Genomes. 6.1 Introduction to Genomics. Genome. Entire complement of genetic information. Includes genes, regulatory sequences, and noncoding DNA. Knowing how many genes determine a phenotype (Mendelian and/or QTL analysis), and where the genes are located (linkage mapping) is a first step in understanding the genetic basis of a phenotype . A . “. User facility. providing high-throughput DNA sequencing & analysis in support of DOE missions in alternative . energy. , . carbon cycling . & . bioremediation. .”. Opened in 1999. ~240 UC Employees. Judith U. Oguzie. , DVM. PhD Research Fellow, World Bank-African Center of Excellence for Genomics of Infectious Diseases. (ACEGID). Redeemer’s University, Ede, Nigeri. a. How?. By establishing a continental consortium of key national laboratories, academic and public health institutions to advance outbreak surveillance and research in Africa. T – Its History and Advancements into New Research and Technology Jutta Marzillier , Ph.D Lehigh University Biological Sciences September 5 th , 2014 Objectives Techniques that enabled genome sequ Update and rationale. Low-pass sequencing (Goncalo). Analysis . Disease projects (Sardinia, T2D). A public database of. . SNPs. . and detectable . CNVs. with allele frequency. >1. % in each of multiple human population samples. Illumina uses photo-cleavable fluorescent nucleotides and terminators for DNA Sequencing . Both groups are cleaved off after recording fluorescence of incorporated nucleotide. Each nucleotide (dATP, dTTP, dGTP, or dCTP) have a different fluorescent group, .