PPT-Module introduction, genomes and sequencing projects

Author : jane-oiler | Published Date : 2019-03-20

Dr Vladimir Teif BS222 Genome Science Lecture 1 Welcome to BS222 Genome Science Meet your lecturers Vladimir Teif module supervisor Leonard Schalkwyk Patrick

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Module introduction, genomes and sequencing projects: Transcript


Dr Vladimir Teif BS222 Genome Science Lecture 1 Welcome to BS222 Genome Science Meet your lecturers Vladimir Teif module supervisor Leonard Schalkwyk Patrick VargaWeisz Jordi Paps. Last lecture summary. recombinant DNA technology. DNA polymerase (copy DNA), restriction endonucleases (cut DNA), ligases (join DNA). DNA cloning – vector (plasmid, BAC), PCR. genome mapping. relative locations of genes are established by following inheritance patterns. Designing. . your. . Sequencing. Run. https://genohub.com/next-generation-sequencing-guide/. Sequencing strategy. Genome size and genome complexity?!. . related organism, PFGE, flow . cytometry. Noncoding. Stefano . Lise. Bioinformatics & Statistical Genetics (BSG) Core. The . Wellcome. Trust Centre for Human Genetics (WTCHG), Oxford. Email: stefano@well.ox.ac.uk. Outline. Human genetic variation in health and disease. Venter et. al (2004). Presented by. Ken . Vittayarukskul. Steven S. White.. Context of the Problem . Evolutionary history is directly tied to microbial genetics. Little is known. Until recently, microbial diversity was measured by PCR amplification and sequencing of only ribosomal genes. 2014 Workshop. G . × . E ANALYSIS. Diego . Jarquin. Chicago, IL. December 10. Variance components for common . inbreds. and hybrids. Genomes to Fields . Inbred – Pollen DAP. Inbred – Plant height. Comparative genomics of sponges, sea anemones, and multicellular pancakes. Mansi Srivastava. Rokhsar Lab, Department of Molecular and Cell Biology, UC Berkeley. Reddien Lab, Whitehead Institute for Biomedical Research. DNA. Double helix. Bases. Sequence. Gene . Key words. Genome . Genome sequencing. Species . Human Genome Project. DNA. is found in the nucleus of a cell. It is a long molecule that carries a set of codes to make you unique.. How we obtain the sequence of nucleotides of a species. …ACGTGACTGAGGACCGTG. CGACTGAGACTGACTGGGT. CTAGCTAGACTACGTTTTA. TATATATATACGTCGTCGT. ACTGATGACTAGATTACAG. ACTGATTTAGATACCTGAC. TGATTTTAAAAAAATATT…. tens of thousands of DNA bases in length. Fugu is thedraft sequenced after human. Its compact form and sim-sequence. We now have in hand the basic gene-leveldescription of two vertebrates. Compari tens of thousands of DNA bases in length Fugu is thedraft sequenced after human Its compact form and sim-sequence We now have in hand the basic gene-leveldescription of two vertebrates Comparing a Circularly Permuted Genomes. Some phages have circularly permuted genomes. This means a linear concatamer of phage DNA is synthesized, used to fill a phage head, then cut when the head is full. Generally, one head will fit more than 100% of a genome, say, 103-110%. This ensures that wherever the DNA is cut, at least one working copy of each gene is present.. DNA Replication Review. Enzymes open . double stranded DNA. Origin of replication. Replication fork. DNA replication in 5’ to 3’ direction. Leading and Lagging Strands. RNA primers. DNA polymerase. Genomic Medicine. Update Session. Provided by Catherine Carpenter-Clawson Programme Manager, WE . GMC with Tracie Miles Genomics Champion RUH, Bath.. Courtesy . of . Genomics England. The Human Genome Project. Skin SSG . May 2018. Catherine Carpenter-Clawson, Programme Manager, WE GMC. Courtesy of Genomics England. From . Genetics . to . Genomics. Genetic testing = single gene. (. s. ). or panels. . Exome sequencing = coding DNA .

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