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 Supplementary Figure 1: Immunostaining  Supplementary Figure 1: Immunostaining

Supplementary Figure 1: Immunostaining - PowerPoint Presentation

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Uploaded On 2020-04-09

Supplementary Figure 1: Immunostaining - PPT Presentation

of TERT protein in human gliomas with TMab6 and sc7215 We immunostained TERT wildtype and mutant gliomas with our newly developed TMab6 and the most widely used commercially available sc7215 Both antibodies detected nuclear ID: 776544

tert case wildtype gliomas tert case wildtype gliomas tmab 7215 mutant c228t oligo astro anaplastic

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Slide1

Supplementary Figure 1: Immunostaining of TERT protein in human gliomas with TMab-6 and sc-7215. We immunostained TERT wildtype and mutant gliomas with our newly developed TMab-6 and the most widely used, commercially available sc-7215. Both antibodies detected nuclear immunoreactitvity of TERT in TERT wildtype and mutated gliomas although the staining intensities were variable. AA, anaplastic astrocytoma; AO, anaplastic oligodendroglioma. Scale bar = 40 μm.

Masui_suppl Fig1

Case: Astro_2

(DA)

TERT

-wildtype

Case: Oligo_1

(AO)

TERT

-

mutant (C228T)

TMab-6

(

0.5 μg/mL)

sc-7215(1.0 μg/mL)

Case: Oligo_10

(AO)TERT-mutant (C228T)

Case: Astro_4

(AA)

TERT

-wildtype