PDF-Blood agar plate with isolated colonies in final streak area

Streaking a Plate for Isolated Colonies Pure Culture Technique Primary Secondary Tertiary Final Streak Streak Streak Streak Streaking a plate for isolation is a method of reducing the nu. numerical aperture (NA); a low resolving power number is good while a high number is bad. Therefore, a shorter wavelength and a higher NA gives better resolving power. The numerical aperture is a prop Enumeration . of yeasts and . moulds. in foods. Fungi kingdom. (yeasts and molds). -. Subkingdom. : based on . septation. of mycelia. Phylum: based on ability to produce and type of sexual spores. The standard plate count method.. Spectrophotometer (turbid metric) analysis.. The standard plate count method is an indirect measurement of cell density ( live bacteria). . The spectrophotometer analysis is based on turbidity and indirectly measures all bacteria (cell biomass), dead and alive.. 1. Operating a microscope and performing a four quadrant streak manual. By: Jaclyn Flores. 2. Table of contents. Introduction……………………….…………………4. Microscope………………………….………………...5. . 1. Label the 5 Agar Plates: . Control. , and the names of the four water samples to be tested. (ie. Urban Treated, Rural Treated, Untreated Raw, Local Treated) 2. Label the 5 sterile pipettes: Control (CW), and the appropriate water sample names (ie. Urban Treated, Rural Treated, Untreated Raw, Local Treated) . Biology & Biotechnology Department. PURPOSE :. Blood agar . is . used for . :. isolation and cultivation of many types of fastidious bacteria. . It is also used to differentiate bacteria based on their hemolytic characteristics, especially within the genera . Staph. , . Strep. and Gram – pathogen part II. LAB . # . 12. Medgar Evers College. Prof. Santos. Exercise 70 week 3. Last week you streaked 2 plates, SM110 and MSA. Today, you will pick isolated colonies from the plates that are characteristic of . Radiant energy released by certain electromagnetic processes . Divided into a spectrum based on wavelength. Longer wavelength: lower in energy. Radio waves, microwaves, infrared . Shorter wavelength then visible light: higher energy level . Biology & Biotechnology Department. PURPOSE :. Blood agar . is . used for . :. isolation and cultivation of many types of fastidious bacteria. . It is also used to differentiate bacteria based on their hemolytic characteristics, especially within the genera . http://www.youtube.com/watch?v=tBmNitxvqyc. Aseptic Technique allows for the sterilization of tools and the prevention of cross-contamination of cultures when working with bacteria as well as the spread of bacteria of and disease to people. These bacteria are . G. +ve. ,. cells aggregation like bunch of grapes, non-motile, non-spore-forming, and facultative anaerobic, colonies are round, convex, . mucoid. . Staphylococci are found on the skin mouth and upper respiratory tract. Such as Staphylococcus . Theory. :. Generally bacteria exist in mixed population. It is very rare to get a single and pure form. For studying the cultural, morphological and physiological characters of an individual species it is essential to separate them from the others to get in the form called pure culture . There are many important methods for isolating pure culture from mixed . Part II. MJ. Weintraub, . DVM. Cell wall/envelope structure . GN. , GP, AF & wall-less. Genome . Morphology, arrangements, capsule/slime layer, flagella, fimbriae, . pilus. Horizontal gene transfer mechanisms. Method of specimen collection. Lumbar puncture (LP), . is . a medical procedure in which a needle is inserted into the . spinal . canal. .. . most commonly to collect . cerebrospinal fluid.  (CSF) for diagnostic testing. .