Incorporating a research project into a required course Fall 2003 Centenary College Fall Sophomore Cell Biology 2 lectures 3 labs Spring Junior Genetics 1 lecture 23 labs and juniorsenior course eg Advanced Cell Bio Cancer Bio ID: 591963
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Slide1
Coupling teaching and research
Incorporating a research project into a required courseSlide2
Fall 2003 Centenary College
Fall: Sophomore Cell Biology (2 lectures, 3 labs)
Spring: Junior Genetics (1 lecture, 2-3 labs) and junior/senior course (e.g., Advanced Cell Bio, Cancer Bio)Slide3
Summer:
Return to
UVa
to do researchSlide4
How can I keep my research going during the academic year? Slide5
Collaborate!
Worked with Dr. Lucy Robinson to develop a course-based undergraduate research experienceSlide6
Our goals
For students
Gain understanding of genetics concepts and molecular biology techniques through using them in the context of a multi-step research project
Learn to use bioinformatics tools
Increased ability to analyze and communicate results of a multi-step projectFor Lucy and meIntegrate my research into my teachingPrepare students for summer and academic year independent research projectsSlide7
The question:
CK1 protein kinases are distinct from other kinase families. Why?Slide8
The approach: Students will identify amino acids that are conserved in CK1s but not in other kinases, locate them in a CK1 model, and form hypotheses about their function.Slide9
Students will design and generate mutations…
Mutagenic primer
pLR10
GFP
Yck2
Amp r
Site-directed mutagenesis on Yck2 CK1
Mutant DNA
Digest wildtype DNA using
Dpn
1 restriction enzyme
pLR10
GFP
Yck2
Amp r
pLR10
GFP
Yck2
Amp r
Transform into
E. coli
and grow selectively on ampicillin plates
Grow colonies in liquid culture to amplify mutant plasmid via replication
Purify GFP-mYck2 plasmid out of
E. coli
culture using a miniprep
pLR10
GFP
Yck2
Amp r
Check plasmid DNA in two ways
b. Sequencing
a. Restriction digest
pLR10
GFP
Yck2
Amp r
pRS315
Amp r
pRS315
Amp r
Restriction digest to cleave out GFP-Yck2
Restriction digest for sticky ends
Ligate GFP-mYck2 into pRS315
pRS315
mYck2
GFP
Amp r
GFP - mYck2
Transform into
E. coli
and grow selectively on ampicillin plates
Grow colonies in liquid culture to amplify plasmid via replication
Purify mYck2-pRS315 construct out of
E. coli
culture
pRS315
mYck2
GFP
Amp r
Transform into Yck
Δ
and Yck
ts
yeast, patch colonies to test functionality
- control
+ control
mutant
Permissive- all patches grow
- control
+ control
mutant
Restrictive- patches only with functional YCK2 growSlide10
…to test their hypotheses.
3 hour blocks
Groups of two students
Groups collaborate to answer question and to replicateSlide11Slide12
Our goals
For students
Gain understanding of genetics concepts and molecular biology techniques through using them in the context of a multi-step research project
Learn to use bioinformatics tools
Increased ability to analyze and communicate results of a multi-step projectFor Lucy and me
Integrate my research into my teaching
Prepare students for summer and academic year independent research projects
Local grants
DBER publicationSlide13
What came next?
Iterative improvement
Experimental implementation
Student assignmentsNew collaboration
Molecular modeling Slide14
What were the lessons?
Collaborate
Iterate
PermutateReplicateCommunicateDelegateSlide15
What didn’t we do well?
Assessment
Joining a network
But you can! CFT Teaching Guide: Introducing research into your courses: summary, examples, references, resources
How-to guide