Onega Ulianova Saratov State Agrarian University RUSSIA Sergey Ulyanov Saratov State University RUSSIA Inactivation of bacteria vaccine strain of the plague microbe Y pestis EV NIIÈG ID: 341684
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Slide1
CULTURAL AND MORPHOLOGICAL PROPERTIES OF THE VACCINE STRAIN OF BACTERIA Y. PESTIS EV NIIEG AFTER PHOTODYNAMIC INACTIVATION
Onega
Ulianova
, Saratov State Agrarian University, RUSSIA
Sergey Ulyanov, Saratov State University, RUSSIASlide2
Inactivation of bacteria vaccine strain of the plague microbe Y. pestis
EV NIIÈG
have
been carried out by photodynamic method (PDT
).
Cells of Y.
pestis
EV NIIÈG after the PDT and intact cells have been cultivated on
bactoagar
at
37
O
C. In
experiments
with
cell
inactivation,
vaccine strain of
Y
.
pestis
EV
have been used, with
concentration of
bacterial
suspension
10
9
microbial cells/ml.
Bacteria have been processed by
radiation of
red LED (
λo
= 650 nm,
Δ
λ
= 10 nm), power density
have been varied at
1, 3 and 5
mW
/cm
2
.
Methylene blue has been added to bacterial
suspension
in very small concentrations
of
0.0005
%,
0.05% and
0.005
%
.
Inactivation
of suspensions have been carried out during 5-360 min.
The
results of experiments are presented in
Figures below.Slide3
Time, min
Time, min
Time, min
Changing of number of colonies-generated cells (KOE)
Y
. pestis
EV
NIEG
(
Осд)
after
photoinactivation
(1 м
W
/
c
m
2
):
exposition with methylene blue
with concentration
0,0005
% (а); 0,005 %
(
b
);
0,05 %
(
c
)
during
5–60
min
;
Кк –
culture control
;
Кмс –
culture control
with
methylene blue
;
Ксд –
culture of illumination by light emitting diodesSlide4
Time, min
Time, min
Time, min
Changing of number of colonies-generated cells (KOE)
Y
. pestis
EV
NIEG
(
Осд) after photoinactivation
(
3
м
W
/
c
m
2
):
exposition with methylene blue
with concentration
0,0005
% (а); 0,005 %
(
b
);
0,05 %
(
c
)
during
5–60
min
;
Кк –
culture control
;
Кмс –
culture control
with
methylene blue
;
Ксд –
culture of illumination by light emitting diodesSlide5
Time, min
Time, min
Time, min
Changing of number of colonies-generated cells (KOE)
Y
. pestis
EV
NIEG
(
Осд) after photoinactivation (
5
м
W
/
c
m
2
):
exposition with methylene blue
with concentration
0,0005
% (а); 0,005 %
(b); 0,05 % (c) during 5–60 min; Кк – culture control ; Кмс – culture control with methylene blue ; Ксд – culture of illumination by light emitting diodesSlide6
Time, 10
4
s
Time, 10
4
s
Time, 10
4
s
Dependence of parameter of growth of cells of
Y
. pestis
EV
(grow)
on time of
photoinactivations
of bacteria
and concentration of methylene blue
0,05
% (а); 0,005 % (б); 0,0005 % (в
)
P=0.2
mW
P=0.6 mWP=1 mWSlide7
Dependence of parameter of growth of cells of
Y. pestis
EV
(grow)
on dose of (J) and concentration of methylene blue 0,05 % (а); 0,005 % (б); 0,0005 % (в)
Dose, JSlide8
Changes of number of
colonies-generated cells
Y. pestis
EV
NIEG
after
photoinactivation
I
= 1мВт/см
2
,
concentration of methylene blue is
0,005
%
Slide9
Changes of number of
colonies-generated cells
Y. pestis
EV
NIEG
after
photoinactivation
I
= 1мВт/см
2
,
concentration of methylene blue is
0,005
%
Slide10
Changes of number of
colonies-generated cells
Y. pestis
EV
NIEG
after
photoinactivation
I
= 1мВт/см
2
,
concentration of methylene blue is
0,005
%
Slide11
Changes of number of
colonies-generated cells
Y. pestis
EV
NIEG
after
photoinactivation
I
= 1мВт/см2, concentration of methylene blue is
0,005 % Slide12
Resume:
It is extremely important to note that both activation and inhibition of cell growth does not depend on the dose
only , but also on the
mode of exposure.
When defining the dependence of light-induced effects in bacterial suspensions of photosensitizer concentration it was quite evident that the concentration of singlet oxygen produced in a bacterial suspension and interacts with cells depends not only on the intensity of light, but also on the initial concentration of photosensitizer.Thus, the product of dose and concentration is an important parameter in determining the processes of interaction of light with bacterial cells. Dependence of parameters of cell growth of Y.
pestis EV (during the process of photoinactivation
) on product of exposure to concentration of methylene blue is characterized by a significant dispersion of the received data.