PPT-Monitoring HLA-specific antibodies

Matthew J Everly PharmD BCPS One Lambda Inc In patients undergoing desensitization Monitoring HLAspecific antibodies Matthew J Everly PharmD BCPS One Lambda Inc In all transplant patients . 6.5 Antibodies . Learning outcomes. Student should be able to understand the following:. Antibody structure and the formation of an antigen-antibody complex. The use of monoclonal antibodies in enabling the targeting of specific substances and cells.. . Lab. 2. Immunoassay. An immunoassay is a test that uses antibody and antigen complexes as a . mean . of generating a measurable . result. Immuno. . refers to an immune response that causes the body to generate antibodies, . The . First. Line of Defence:. Primarily physical defence. Includes:. - . The Skin. . - Lysozymes . . . (antimicrobial enzymes in tears, sweat, saliva, & mucous). . - . Mucous and Cilia in Respiratory Tract. Immune Responses. Applications of the Immune Response. A. Vaccination – the practice of deliberately stimulating the immune system in order to protect individuals against a disease. 1. Edward Jenner developed the first official smallpox . Purpose of the Immune System . Protect from internal attacks from . abnormal body cells . (cancerous cells). Protect from . external pathogens. Can enter the body through any systems that have openings on the exterior of the body. cELISA. for the detection of FMDV/A antibodies . Dr. M. Yang . National Center for Foreign Animal diseases. /Canadian Food Inspection Agency. Introduction . Foot-and-mouth disease (FMD) . remains one of the world’s . mmuno. s. orbant . A. ssay. Detects the presence of minutes quantities of either an antibody or an antigen. Important diagnostic tool in many areas of science and industry. 1. Basics of ELISA. The solution to be tested is “unknown” in that it might or might not contain the substance (antigen) of interest.. THE IMMUNE RESPONSE AND IMMUNITY. Immune response. Innate (non-specific). Adaptive or Acquired (specific). Defense . Mechanisms. Nonspecific. defense mechanisms. Specific. defense mechanisms. (immune. Iraqi Center for Cancer and Medical Genetics Researches. Application of ELISA. Presence of antigen or the presence of antibody in a sample can be evaluated.. Determination of serum antibody concentrations in a virus test.. Martin Liška. . Department . of. . Immunology. . and. . Allergology. . Faculty. . of. . Medicine. . and. . Faculty. . Hospital. in . Pilsen. Topics. :. Laboratory methods of assessment of . - . ontogenesis. , . surface. . markers. , . function. . . . Ontogenesis. . of. . antibody. . production. .    . 16. . Immunoglobulins. (. structure. , . classes. ). . Genetic. . background . Pharmacology III. Immunosuppressive drugs are useful in: . a) Treating autoimmune diseases; and . b) Preventing . immunorejection. of organ transplants.. Immunosuppresants. may be classified as-. Calcineurin inhibitors: Cyclosporine Tacrolimus . Learning outcomes. Student should be able to understand the following:. Antibody structure and the formation of an antigen-antibody complex. The use of monoclonal antibodies in enabling the targeting of specific substances and cells.. Parasitology. University. of Copenhagen. Utilizing. . nanobody. . technology. to . target. non-. immunodominant. domains of . VAR2CSA. P. . falciparum. P. . falciparum. . Erythrocyte. . Membrane.