Escherichia coli Becca Goslow WMHS Frosilda Pushani SHHS Did You Know 2 http thescienceofacnecom amoxicillin https harveymillicanwordpresscom tagamoxicillin ID: 297627
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Slide1
Effect of Temperature and Ampicillin on Escherichia coli
Becca
Goslow
- WMHS
Frosilda
Pushani
- SHHS Slide2
Did You Know…?
2
http://
thescienceofacne.com
/amoxicillin/
https://
harveymillican.wordpress.com
/tag/amoxicillin/
Goslow-PushaniSlide3
Watch This!
3
https://
www.youtube.com
/
watch?v
=
RpKZvnJwicAGoslow-PushaniSlide4
Terms to knowAntibiotic ResistanceEscherichia coliAmpicillinInhibition Zone
Goslow-Pushani
4Slide5
What is Antibiotic Resistance?Antibiotics are no longer an option.Bacteria takes over and becomes immune.
Goslow-Pushani
5
http://
www.medicinenet.com
/
antibiotic_resistance
/page4.htmSlide6
Escherichia coli6
http://
www.britannica.com
/blogs/2011/06/evil-coli/
http://www.health32.com/e-coli-symptoms-and-treatment/
Goslow-PushaniSlide7
Ampicillin7
www.newlystar-medtech.com
/Ampicillin-Capsules-12.html
http://
www.drugs.com
/cdi/
ampicillin.html
Goslow-PushaniSlide8
Inhibition Zones8
http://
web.med.unsw.edu.au
/
cdstest
/
gtf_cds_site/webpages/plates/plate1a.htm
Goslow-PushaniSlide9
OverviewProblem/Hypothesis.Materials/Procedure.Results/Statistics.
Accept/Reject Hypothesis.
Possible Improvements.
9
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ProblemThe effect of antibiotics on the growth of bacteria throughout generations.
10
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HypothesisIf Ampicillin is used, then the Escherichia coli will develop a resistance to the antibiotic.
11
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Materials12
Blank Disks
Ampicillin
Sterile Swabs
Petri Dishes
Starter Plate (
E. coli
)
Bunsen Burner
Analytic Balance
Incubators
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ValuesGoslow-Pushani
13Slide14
Procedure14
https://www.youtube.com/watch?v=cneascR3OEc
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Procedure15
https://www.youtube.com/watch?v=bRadiLXkqoU
Goslow-PushaniSlide16
Procedure16
http://
en.wikipedia.org
/wiki/Capsule_(pharmacy)
http://
www.carolina.com
/microbiology-supplies/antibiotic-sensitivity-disks-blank-sterile-vial-50/805091.pr
Goslow-PushaniSlide17
Procedure17
http://out-
grow.com
/cultivation-equipment-c-1/
http://
www.ufz.de
/
iwas-sachsen
/
index.php?en
=21853
Goslow-PushaniSlide18
Procedure18
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Procedure
(A
+
B
+
C)
÷ 3
= Size of an inhibition zone.
19
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ResistanceE. coli.Ampicillin.
Zone of Inhibition/ Resistance.
20
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Results21
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Observations
Date
Observation
3/
11/
14
One Petri dish had a smaller
inhibition
zone
from the rest. The
E. coli
was smelly and white.
3/13/14
The Petri dishes had a lot of
E. coli
, the bacteria was white and extremely smelly.
3/14/14
The
E. coli
on some of the Petri dishes were blotchy.
3/18/14
The
E. coli
was smellier than usual and filled up every Petri dish completely, besides the zone of inhibition.
22
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Effect of Ampicillin23
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Effect of Incubation Temperature24
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Interaction Effect25
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Outliers?26
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Accepted
Hypothesis!
27
Goslow-PushaniSlide28
28
Goslow-Pushani
Accepted
Hypothesis!Slide29
RecapWhat we planned.What happened.Added Ampicillin to E. coli and incubated the petri dishes.
The bacteria and inhibition zones grew.
Overtime, the inhibitions decreased in size.
The hypothesis was accepted!
29
Goslow-PushaniSlide30
Improvements for the FutureRun more trials.Have a wider range of the predictor variables.Use of an actual human.
Stop experimental flaws.
30
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Flaw and Human ErrorsOff temperatures.Off measurements of Ampicillin.Time issues.
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31Slide32
Who does this effect?Chemists/ScientistsDoctorsYOU!
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32Slide33
Save Your LifeDo not take the antibiotics for viral infections.Take an antibiotic exactly as a healthcare provider tell you.Keep antibiotics to a minimum.
33
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AcknowledgementsMr. AcreMr. Estapa
Mrs. Gravel
Veronica & Abigail
Goslow-Pushani
34Slide35
Works Cited35
"Ampicillin Capsules."
Ampicillin Capsules
. Web. 28 May 2014
.
"Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com
." Ampicillin Capsules: Indications, Side Effects, Warnings - Drugs.com. Web. 27 May 2014.
"Antibiotic Resistance." Antibiotic Resistance. University of Utah Health Sciences, n.d. Web. 14 May 2014.
"Antibiotic Resistance a Serious Threat." The Australian Government,
n.d.
Web. 10 May 2014.
"Antibiotic Resistance Questions & Answers."
Centers for Disease Control and Prevention
. Centers for Disease
Control
and Prevention, 18 Dec. 2013. Web. 10 May 2014.
"Antibiotic Sensitivity Disks, Blank, Sterile, Vial 50."
Science Supplies & Curriculum
. Web. 28 May 2014
.
"Aseptic Technique."
YouTube
. YouTube. Web. 28 May 2014
.
"Capsule (pharmacy)."
Wikipedia
. Wikimedia Foundation, 24 May 2014. Web. 28 May 2014
.
"Cultivation Equipment."
: Out-Grow, Mushroom Growing Supplies & Quality Service
. Web. 28 May 2014
.
Dzidic
,
Senka
. "Antibiotic Resistance Mechanisms in Bacteria: Biochemical and Genetic Aspects."
Food Technology
and
Biotechnology
.
N.p
.,
n.d.
Web. 15 Mar. 2014.
Goslow-PushaniSlide36
Works Cited"E.coli
Symptoms and Treatment."
Health32Com
Ecoli
Symptoms and Treatment Comments.Web. 28 May
2014."Evil E. Coli." Encyclopedia Britannica Online
. Encyclopedia Britannica. Web. 28 May 2014."HowStuffWorks
.” "How Do Antibiotics Work?"" HowStuffWorks. N.p., n.d. Web. 26 May 2014
.
"International Water Research Alliance Saxony."
Running Waters, Microbiology -
. Web. 28 May 2014
.
"Media Prep."
YouTube
. YouTube. Web. 28 May 2014
.
Paphitou
,
Niki
I. "Antimicrobial Resistance: Action to Combat the Rising Microbial Challenges
.”
International
Journal of
Antimicrobial
Agents
.
N.p
.,
n.d.
Web. 15 Mar. 2014.
"Plate1A."
Plate1A
. Web. 27 May 2014
.
"Science Fair Projects - Bacteria resistance to antibiotics."
Science Fair Projects - Bacteria resistance to
antibiotics
. All
Science
Fair
Projects
,
n.d.
Web. 14 May 2014.
"The Science of Acne."
The Science of Acne
. Web. 28 May 2014
.
"Tag Archives: Amoxicillin."
Harvey
Millican
Raising Your Kids Without Lowering Your IQ
.
Web
. 28 May 2014
.
"The Threat of Antibiotic Resistance."
YouTube
. YouTube. Web. 28 May 2014
.
Todar, Kenneth . "Bacterial Resistance to Antibiotics."
Bacterial Resistance to Antibiotics
.
N.p
.,
n.d.
Web. 14
May
2014.
36
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Any Questions?37
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38Goslow-Pushani