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Innovative Analytical Solutions for the Dairy Industry Innovative Analytical Solutions for the Dairy Industry

Innovative Analytical Solutions for the Dairy Industry - PowerPoint Presentation

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Uploaded On 2017-07-04

Innovative Analytical Solutions for the Dairy Industry - PPT Presentation

Bentley CombiFTS Rapid and accurate determination of milk chemical composition and somatic cells content Milchprüfring BadenWürttemberg Germany 5xCombiFTS 500 1 IBC150 Bentley Bartec CombiFTS 600 ID: 566577

milk bhb bentley µg bhb milk µg bentley blood combifts log samples 600 lfref acids ibc µl infrared lfir

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Slide1

Innovative Analytical Solutions for the Dairy IndustrySlide2

Bentley CombiFTS

Rapid and accurate determination of milk chemical composition and somatic cells content

Milchprüfring

Baden-Württemberg, Germany

5xCombiFTS 500, 1 IBC150Slide3

Bentley Bartec CombiFTS 600

Up to

600

samples/hrSlide4

Bentley Bartec CombiFTS 500Slide5

Bentley ILAS CombiFTS 600

Up to

600

samples/hrSlide6

Bentley ILAS CombiFTS 600

The Optimum Solution

Fully Automated

W

armed up in a water bath, shaken and identified (RFID, Barcode)

U

ncapped

Samples are replaced in the tray after analysis

Pilot samples automatically inserted in the processSlide7

Bentley CombiFTS

Somacount FCM (Flow Cytometry)

Bentley FTS (FTIR)Slide8

Bentley FTS

Milk Component Absorption Wavebands

Protein (NH)

Fat A (C=0)

Fatty Acids

Fat B (CH)

Lactose (OH)

Frequency of infrared radiation absorbed depending upon type of molecular bonds /vibration mode Slide9
Slide10
Slide11

Recordings from the Instrument

Infrared Spectrum

Somatic cells peak distributions

Environmental variables could affect the end resultsSample, water bath, ambient, homogenizer, deck temperaturesOptical deck Moisture level and pressure

Centerburst energy level (automatic alignment)Homogenizar pressureVibrationsSlide12

FTS Infrared Applications

Traditional Components

Citric Acid

CaseinpH

FFAFatty Acids ProfileKetone Bodies

New Developments

Blood BHB

Lactoferrin

Iodine Value

Fatty Acids calibrations

New Components

Fat

Protein

Lactose

Solids

SNF

MUN

FDPSlide13

Blood BHB

Monitoring of the cows metabolic activity and detection of ketosis by infrared spectroscopy

Why predict Blood BHB instead of Milk BHB?

Milk BHB is unstable and levels very low

Milk BHB content will decrease rapidly if the milk is not stored at 4°C right after milkingSlide14

Blood BHB

Calibration on 696 cow samplesSlide15

Blood BHB

Correlation spectrum between

Blood BHB content &

milk spectra

The spectral zone with the highest correlation are related to the milk fatty acids profileSlide16

Lactoferrin

Used for early detection of mastitis & monitoring of the sanitary status of the mammary gland

Iron binding antimicrobial glycoprotein

Plays a role in pre-immune host defense

Strong association between LF & occurrence of mastitis

Used to check sanitary status of the mammary glands in the herdSlide17

Lactoferrin

 

LFir

(

µg/

mL

)

LFref

(

µg/

mL

)

Log

LFir

(µg/

mL

)

Log

LFref

(µg/

mL

)

IBC/µL

Log IBC/µL

SCC/µL

Log SCC/µL

Healthy

144,04

120,85

1,86

1,99

314

2,34

95

1,88

Accute

210,32

234,46

2,09

2,33

5592

3,53

1967

3,19

Chronic

226,98

250,66

2,15

2,38

8273

3,74

2863

3,31

Min

41

15

1,13

1,17

63

1,80

11

1,04

Max

413

412

2,45

2,62

22082

4,34

9999

4,00

Mean

194

202

2,04

2,23

4726

3,20

1642

2,79

Sd

66,02

99,03

0,24

0,28

6213,44

0,74

2242,39

0,72

LFir

(

µg/

mL

)

LFRef

(µg/

mL

)

logLFir

(µg/

mL

)

LogLFRef

(µg/

mL

)

IBC/µL

Log IBC/µL

SCC/µL

Log SCC/µL

Sy,x/

LFref

77,13

 

0,21

 

0,26

0,22

0,25

0,21

r/Log

LFRef

 

 

0,65

1,00

0,42

0,64

0,43

0,68Slide18

Lactoferrin