PPT-HPLC High Performance Liquid Chromatography

WJEC IVQ Science for Work Unit 3 WJEC Pathways Science for Work Unit 28 WJEC GCSE Additional Applied Science Diagram HPLC solvent reservoir pump detector waste injection port HPLC. By: Maria Santos. Nancy Zepeda. Introduction. . What is ion-pair . chromatography (IPC)?. T. he . addition of an ionic surfactant to a reversed-. phase Chromatography system (RPC) . in order to affect retention . Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with What is HPLC?. Types of Separations. Columns and Stationary Phases. Mobile Phases and Their Role in Separations. Injection in HPLC. Detection in HPLC. Variations on Traditional HPLC. Ion Chromatography. #Certification #Dumps #Certification_exam_Dumps
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Certification dumps are collections of questions and answers used to prepare for a certification exam. They are often provided by third-party companies that specialize in exam preparation. Certification dumps are a valuable resource for anyone looking to prepare for a certification exam, as they provide an in-depth overview of the topics and concepts covered on the exam. Additionally, they are often updated with new and relevant information to ensure that the material is as fresh and up-to-date as possible. Certification dumps can save time and money by providing a comprehensive and convenient way to prepare for a certification exam . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . By . Dr. . Amjad. M. T. Khan. Assistant Professor. Department of Chemistry. AMU, Aligarh. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. . . CHROMATOGRAPHIC TECHNIQUES. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. Adsorption chromatography. Partition chromatography.