PPT-Smooth Muscle Actin (SMA1) Stain Lungs and Heart on Strains of CUX-1 Transgenic Mice

S Dasari 1 S Kesh 1 J Buursma 2 S Livingston 2 D Kearn 2 B Herndon 1 G Vanden Heuvel 3 R Baybutt 2 A Molteni 1 1 UMKC School of Medicine 2 Wheaton College . Excitation - Contraction. Mike Clark, M.D.. Smooth Muscle. Found in walls of most hollow organs. (except heart). Usually in two layers (longitudinal and circular). Figure 9.26. Small. intestine. (a). Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. Dr. . Ayisha. . Qureshi. . MBBS, MPhil. Assistant Professor. CLASSIFICATION OF SMOOTH MUSCLES. UNITARY/ SINGLE UNIT/SYNCYTIAL/VISCERAL. Muscles of visceral organs .e.g. GIT, uterus, ureters & some of the smaller blood vessels.. We have previously characterized the changes in. duced by this gene in . kidney development, spermatogenesis, and liver development in Cux1 transgenic mice. . In this study, we . report. the changes in the lung resulting from constitutive Cux1 expression in transgenic mice.. Histology Lecture . Series. Larry . Johnson, Professor. Veterinary Integrative Biosciences. Texas A&M University. College Station, . TX 77843. Muscle. – Introduction. Contractivity. is one of the . pull. , not . push. . And it needs certain parts to do this.. Microscopic anatomy of muscle. The bits and pieces. Sarcolemma. – plasma membrane of a muscle cell. Myofibrils. – long ribbon-like organelles that take up the majority of the space in a muscle cell. 1. Frontal lobe: . encodes and manipulates action memories.. 2. Dorsal cortex . (. occipital, parietal, . and. temporal lobes. ). : stores . encoded sensory memories.. Frontal. lobe. Parietal. lobe. Chapter 12 – Transgenic Animals Transgenic mice: methodology Transgenic mice: applications Transgenic livestock Transgenic poultry Transgenic fish Establishing transgenic mice by DNA microinjection matt.velkey@duke.edu. 452A Davison, Duke South. Muscle Tissue. I. Striated Muscle - regularly arranged contractile units. A. Skeletal Muscle - long, cylindrical multinucleated cells with peripherally placed nuclei. Contraction is typically quick and vigorous and under voluntary control. Used for locomotion, mastication, and phonation.. Dr. Kamal Kumar Gupta. Mice from Dr. Ralph L. . Brinster's. Giant . M. ouse Experiment . T. he human . growth hormone gene was expressed in the liver of mice. Knockout Mouse. A . mouse in which a gene affecting hair growth has been knocked . soft tissue. found in most animals. . Muscle cells. contain . protein. filaments of . actin. and . myosin. that slide past one another, producing a . contraction. that changes both the length and the shape of the cell. Muscles function to produce force and . Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 October 2022 Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 Oct Imre Kacskovics, CEO & CSO. FcRn. : . the . immune response booster. The . neonatal. . Fc. receptor (FcRn). FcRn-IgG . interaction. FcRn. mediates. . maternal. IgG . transport. protect. s. IgG and albumin . Prof. Dr. . Anna L. Kiss . Department. of . Anatomy. , . Histology. and . Embryology. Semmelweis University. Budapest . 2021. Muscle. . tissue. Function. : . contraction. Origin. : . mesoderm. Structure.