PPT-TRANSGENIC ANIMALS 1. CREATION

KAMAL KUMAR GUPTA Transgenic Animals Dolly What is a transgenic animal A transgenic animal is one whose genome has been changed to carry genes from other species transgenes are functionally expressed and stably inherited in the transgenic animals. I. Selective Breeding. 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. - transgenic and non transgenic cotton on arthropod diversity, seed cotton yield and bollworms control M.K. Dhillon 1 , and H.C. Sharma 2 * * 1 Division of Entomology, Indian Agricultural Research Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. Animals - . Technology and applications. Goetz Laible, . AgResearch. Biology Teachers. Professional Development day. 28. th. March 2013. Presentation outline. International TG livestock examples. Historical perspective. : . Insights into . Pharmaceutical Biotechnology and Molecular Farming. Huma. . Mehreen. . Sadaf. 10-arid-1824. Contents:. Background. History of Medicinal Plants. Plant Molecular farming. Potential Benefits Claimed For PMF. chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . Biomedical Technology I. 3.03. GMO’s:. Also known as Transgenic Organisms. Are species that carry one or more genes from another species.. These have continued to improve since the 1970’s. Advantages:. VIF-ICRISAT National Symposium on . Doubling . Farmers’ Income through Scaling-up . 16-3-2017. Nagendra Singh. National Professor ICAR. Outline. Proven Plant Biotechnologies. Plant Tissue Culture. Genomics and Marker-assisted breeding. modified organisms (plants). Genetically engineered . plants)) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . Examples: . Glyphosate herbicide resistance in soybean, corn . fragmentDetermining Transgene Copy Number and Number ofIntegration Sites in Transgenic MiceThom Saunders. University of Michigan Transgenic Core. http://www.med.umich.edu/tamc Assumption: the Haploid Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 October 2022 Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 Oct Agrobacterium. -mediated . transformation. Transgenic Plants. Transgenic plants are those plants, which carry additional, stably integrated and expressed foreign gene from trans species.. A genetically modified organism (GMO) is an animal, plant, or microbe whose DNA has been altered using genetic engineering techniques..