PPT-TRANSGENIC ANIMALS

TRANSGENIC ANIMALS INTRODUCTION OBJECTIVES OF GENE TRANSFER VECTORS Methods of modified dna insertion Transgenic animals INTRODUCTION A TRANSGENIC ANIMAL CONTAINS IN ITS GENOME OR GENES INTRODUCED BY ONE OR THE OTHER TECHNIQUES OF TRANSFECTION. I. Selective Breeding. 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. - transgenic and non transgenic cotton on arthropod diversity, seed cotton yield and bollworms control M.K. Dhillon 1 , and H.C. Sharma 2 * * 1 Division of Entomology, Indian Agricultural Research Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. : . Insights into . Pharmaceutical Biotechnology and Molecular Farming. Huma. . Mehreen. . Sadaf. 10-arid-1824. Contents:. Background. History of Medicinal Plants. Plant Molecular farming. Potential Benefits Claimed For PMF. Idaho Rangeland Resource Commission. Pronghorn Antelope. Pronghorn Antelope. Pronghorns live in the sagebrush grasslands of Idaho.. They have a showy coat that is tan to reddish brown across the back, while the under-parts, including the flanks and rump are white. I have two throat bands, the lip and cheeks, and a spot under each ear are also white.. S Dasari. 1. ,. . S Kesh. 1. , J Buursma. 2. , S Livingston. 2. , D Kearn. 2. ,B Herndon. 1. , G . Vanden. Heuvel. 3. , R Baybutt. 2. , A Molteni. 1. .. 1. UMKC School of Medicine, . 2. Wheaton College, . chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . Chapter 12 – Transgenic Animals Transgenic mice: methodology Transgenic mice: applications Transgenic livestock Transgenic poultry Transgenic fish Establishing transgenic mice by DNA microinjection Applications of Genetic Engineering. Objectives. ____________________the usefulness of some transgenic organisms to humans.. Describe. Transgenic Organisms. An organism described . as_______________, . Dr. Kamal Kumar Gupta. Mice from Dr. Ralph L. . Brinster's. Giant . M. ouse Experiment . T. he human . growth hormone gene was expressed in the liver of mice. Knockout Mouse. A . mouse in which a gene affecting hair growth has been knocked . VIF-ICRISAT National Symposium on . Doubling . Farmers’ Income through Scaling-up . 16-3-2017. Nagendra Singh. National Professor ICAR. Outline. Proven Plant Biotechnologies. Plant Tissue Culture. Genomics and Marker-assisted breeding. fragmentDetermining Transgene Copy Number and Number ofIntegration Sites in Transgenic MiceThom Saunders. University of Michigan Transgenic Core. http://www.med.umich.edu/tamc Assumption: the Haploid Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 October 2022 Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 Oct Introduction. Examples of Transgenic Animals. Finite Cell Lines. 1. 3. Creating Transgenic Animals. 2. Few Questions????. What is a Transgenic Organism. ?. A transgenic organism is one which contains novel heritable genetic material.