PPT-Transgenic Plants: Creation

Dr Kamal Kumar Gupta Associate Professor Methods of production of Transgenic plants M icroprojectile bombardment shooting DNAcoated tungsten or gold particles into plant cells E lectroporation. Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. Animals - . Technology and applications. Goetz Laible, . AgResearch. Biology Teachers. Professional Development day. 28. th. March 2013. Presentation outline. International TG livestock examples. Historical perspective. : . Insights into . Pharmaceutical Biotechnology and Molecular Farming. Huma. . Mehreen. . Sadaf. 10-arid-1824. Contents:. Background. History of Medicinal Plants. Plant Molecular farming. Potential Benefits Claimed For PMF. Plants. (Genetically . modified organisms (plants). Genetically engineered . plants)) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . Chapter 12 – Transgenic Animals Transgenic mice: methodology Transgenic mice: applications Transgenic livestock Transgenic poultry Transgenic fish Establishing transgenic mice by DNA microinjection Genetic manipulation strategies in environmental biotechnology Presented By : * shahzaib khan *WAQAR ALI Contents What is Environmental Biotechnology Genetic Manipulations strategies In Plants In Animals Applications of Genetic Engineering. Objectives. ____________________the usefulness of some transgenic organisms to humans.. Describe. Transgenic Organisms. An organism described . as_______________, . Dr. Kamal Kumar Gupta. Mice from Dr. Ralph L. . Brinster's. Giant . M. ouse Experiment . T. he human . growth hormone gene was expressed in the liver of mice. Knockout Mouse. A . mouse in which a gene affecting hair growth has been knocked . VIF-ICRISAT National Symposium on . Doubling . Farmers’ Income through Scaling-up . 16-3-2017. Nagendra Singh. National Professor ICAR. Outline. Proven Plant Biotechnologies. Plant Tissue Culture. Genomics and Marker-assisted breeding. Imre Kacskovics, CEO & CSO. FcRn. : . the . immune response booster. The . neonatal. . Fc. receptor (FcRn). FcRn-IgG . interaction. FcRn. mediates. . maternal. IgG . transport. protect. s. IgG and albumin . 1. 2. 3. 4. Ti Plasmid & T-DNA transfer. . Herbicide . Resistant . Insect Resistant . Drought/Stress resistant. Delayed . ripening. 5. Gene transfer in plants. Viral . transduction. Major Four.