PPT-Introduction to Yeast Plasmids

Doug Wassarman PUBS Sept 19 2017 What is a plasmid Small DNA molecule Independent replication Circular Used for horizontal gene transfer Contain genes for drug resistance virulence factors etc. Michelle . Attner. July 24, 2012. What is budding yeast, . S. . cerevisiae. ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter). Indispensable tools that allow molecular biologists to obtain essentially unlimited amounts of a DNA sequence. S. mall . circular DNA molecules that replicate independently of the host chromosomes. How are plasmids constructed?. YSC1021 Yeast: Yeast Knock Out StrainYSC4300Yeast Heterozygous (plates 281-282)YSC1053Yeast MATa CollectionYSC4343Yeast Heterozygous Plate 282YSC4298Yeast MATa (plates 72-73)YSC4508Yeast Heterozygous Introduction to Mycology. Mycology is the study of fungi –. Yeast. Mold. Mycology. Yeasts and molds have different structural and reproductive characteristics. Yeast are unicellular, nucleated rounded fungi while molds are multicellular, filamentous fungi. Dr. . Savita . Kumari. Assistant Professor-cum-Jr. Scientist. Department of Veterinary Microbiology. Bihar Veterinary College, BASU, Patna. Veterinary Microbiology (Unit-1). Usually . circular, double stranded extrachromosomal . th the total amount of DNA in a human cell)ll contains small, circular, extrachromosomal (outside of the chromosome) DNA molecules called genetic information. Some plasmids repli Fungi Classification, Morphology and Pathogenicity • Mykes (Greek word) : Mushroom • Differ from bacteria and other prokaryotes : 1. Cell walls containing chitin (rigidity & support), mannan & S. mall . circular DNA molecules that replicate independently of the host chromosomes. How are plasmids constructed?. What functional elements are found in our yeast overexpression plasmids?. How are plasmids purified?. oriC. . gene, along with a . dnaA. . gene and other genes typical of the chromosomal origin of replication.. Plasmids usually carry genes for proteins that are necessary or beneficial to the host under some situations but are not essential under all conditions. . a. . Rolls. b. . Loafs. c. . Doughnuts. Ingredients . Yeast. Flour. Liquids. Flour. Gluten for elasticity & structure.. Source . of starch . When . mixed with liquid and kneaded, the flour develops gluten which supports the carbon dioxide produced by the yeast. . Part 4: How do we isolate the plasmid DNA from our bacteria . Isolating plasmid DNA. The key is to separate the plasmids from the bacterial chromosome. This is not trivial, since they are both DNA. What is the difference?. Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . Cottell JL, Webber MA, Coldham NG, Taylor DL, Cerdeño-Tárraga AM, Hauser H, et al. Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14. Emerg Infect Dis. 2011;17(4):645-652. https://doi.org/10.3201/eid1704.101009. Single celled, and easy to grow like bacteria. Yeast strains are genetically well characterized. Detailed genetic maps are. available for . Saccharomyces. . cerevisiae. and . Schizosaccharomyces. .