PPT-Introduction to Yeast Plasmids

Doug Wassarman PUBS Sept 19 2017 What is a plasmid Small DNA molecule Independent replication Circular Used for horizontal gene transfer Contain genes for drug resistance virulence factors etc. Michelle . Attner. July 24, 2012. What is budding yeast, . S. . cerevisiae. ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter). SDS-PAGE gives a snapshot of proteins in an extract. Proteins are extracted from cells. There are major challenges in analyzing yeast cell proteins!. How can proteins be efficiently extracted without being degraded? . Nanopore. Sequencing of Transmissible . Tetracyline. Plasmids Captured without Cultivation from Stream Sediment Reveals Linked Genes Encoding Resistance to Multiple Human Clinical Antibiotics . J. B. Herrick*, . Chris White and . Jamil. . Zainasheff. San Diego California. June, 2011. Yeast. Chapters. Part One: The Importance of Yeast and Fermentation. Part Two: Biology, Enzymes, and Esters. Part Three: How to Choose the Right Yeast. Indispensable tools that allow molecular biologists to obtain essentially unlimited amounts of a DNA sequence. S. mall . circular DNA molecules that replicate independently of the host chromosomes. How are plasmids constructed?. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. Yeast . yeast (. Saccharomyces. . cerevisiae. ).. yeast is a living organism that requires a warm, moist environment and a food source to grow and thrive. .. unicellular, . faculative. anaerobic fungi. It typically exists as a covalently closed circular piece of double stranded DNA that has the capability of replicating autonomously and it is this property that leads to its isolation and physical recognition. The closed covalent nature of their structure allows them to be separated from chromosomal DNA by either gel electrophoresis or . Plasmid . What is the plasmid ?. Plasmid are small, double strand , closed circular DNA molecules , that can be isolated from . bacterial cells. , they replicate independently of the bacterial chromosome. .. Objective . 1: . Identify and prepare yeast breads. a. Lean Dough, Enriched Dough. b. Mixing Methods: Straight dough, Modified straight-dough, Sponge method.  . Objective 2: . Identify and discuss the different types of flours used in bread making: all-purpose flour, unbleached flour, bread flour, and whole wheat flour.. Why Plasmids?:. Addition of alien bar-code permits identification in a mixture. Multiple plasmids can be mixed into the background of a human reference genome providing controls for multiple targets and sequence variants (missense, in/. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. 1 S.B. Primrose, Molecular Biotechnology, 2 Why Gene Cloning is Important?Why Gene Cloning is Important?A century ago, A century ago, Mendel :Mendel :property of an organism) is property of an organis and phages . and are known as . phasmids. or, if they . contain . an . M13 . ori region. , phagemids. .. 1. . Phagemid. . vectors are plasmids having a small segment of a filamentous phage M-13, . fd.