PPT-Plasmids

Indispensable tools that allow molecular biologists to obtain essentially unlimited amounts of a DNA sequence S mall circular DNA molecules that replicate independently of the host chromosomes How are plasmids constructed. CLS 311: Basic Bacteriology. Mrs. . Amany. Ahmed . Niazy. Bacterial Structure. Bacterial Structure. Bacterial. Structure. Exterior. Structures (cell envelope). Capsule. Cell Wall. Cell Membrane. Filamentous ProteinAppendages. PLASMID VECTORS. . . Cloning into a Plasmid. Bacteria are useful hosts.. They are easily grown. They are cheap to grow. They grow fast. They are easily manipulated in the laboratory. DNA can be inserted - transformation. BY: Selena & Nicole. Foundations of biotechnology . Biotechnology can be defined as the manipulation of living organisms or their components to provide products or to serve useful purpose. As result the DNA of plants, animals and other organisms can be manipulated in the laboratory .. It typically exists as a covalently closed circular piece of double stranded DNA that has the capability of replicating autonomously and it is this property that leads to its isolation and physical recognition. The closed covalent nature of their structure allows them to be separated from chromosomal DNA by either gel electrophoresis or . Plasmid . What is the plasmid ?. Plasmid are small, double strand , closed circular DNA molecules , that can be isolated from . bacterial cells. , they replicate independently of the bacterial chromosome. 1. 16. Copyright © McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display.. Mutations: Their Chemical . Basis and Effects. Stable, heritable changes in sequence of bases in DNA. CLS 212: Medical Microbiology. Miss . Zeina. . Alkudmani. Prokaryotes. Prokaryotic cells possess simpler structures than eukaryotic cells. . , since they . do not have a nucleus or other . cytoplasmic. Why Plasmids?:. Addition of alien bar-code permits identification in a mixture. Multiple plasmids can be mixed into the background of a human reference genome providing controls for multiple targets and sequence variants (missense, in/. A vehicle used to transfer genetic material such as DNA sequence from the donor organism to target cell of recipient organism(Host cell).. Also referred as carrier of DNA sequence.. Also referred as cloning vehicle or cloning DNA.. S. mall . circular DNA molecules that replicate independently of the host chromosomes. How are plasmids constructed?. What functional elements are found in our yeast overexpression plasmids?. How are plasmids purified?. oriC. . gene, along with a . dnaA. . gene and other genes typical of the chromosomal origin of replication.. Plasmids usually carry genes for proteins that are necessary or beneficial to the host under some situations but are not essential under all conditions. . Part 4: How do we isolate the plasmid DNA from our bacteria . Isolating plasmid DNA. The key is to separate the plasmids from the bacterial chromosome. This is not trivial, since they are both DNA. What is the difference?. and phages . and are known as . phasmids. or, if they . contain . an . M13 . ori region. , phagemids. .. 1. . Phagemid. . vectors are plasmids having a small segment of a filamentous phage M-13, . fd. Cottell JL, Webber MA, Coldham NG, Taylor DL, Cerdeño-Tárraga AM, Hauser H, et al. Complete Sequence and Molecular Epidemiology of IncK Epidemic Plasmid Encoding blaCTX-M-14. Emerg Infect Dis. 2011;17(4):645-652. https://doi.org/10.3201/eid1704.101009.