PPT-Transgenic Plants (Genetically

modified organisms plants Genetically engineered plants Why create transgenic plants When there is no naturally occurring genetic variation for the target trait   Examples  Glyphosate herbicide resistance in soybean corn . Plants. (Genetically . modified organisms (plants). Genetically engineered . plants) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . I. Selective Breeding. 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. Traditional and Transgenic Methods and Applications for Insect Resistance in Plants. Some history. 1792: ‘Underhill’ wheat reported to show some resistance to Hessian fly. 1830s: ‘Winter . Majetin. - transgenic and non transgenic cotton on arthropod diversity, seed cotton yield and bollworms control M.K. Dhillon 1 , and H.C. Sharma 2 * * 1 Division of Entomology, Indian Agricultural Research Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. Plants. (Genetically . modified organisms (plants). Genetically engineered . plants)) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. S Dasari. 1. ,. . S Kesh. 1. , J Buursma. 2. , S Livingston. 2. , D Kearn. 2. ,B Herndon. 1. , G . Vanden. Heuvel. 3. , R Baybutt. 2. , A Molteni. 1. .. 1. UMKC School of Medicine, . 2. Wheaton College, . Chapter 12 – Transgenic Animals Transgenic mice: methodology Transgenic mice: applications Transgenic livestock Transgenic poultry Transgenic fish Establishing transgenic mice by DNA microinjection Applications of Genetic Engineering. Objectives. ____________________the usefulness of some transgenic organisms to humans.. Describe. Transgenic Organisms. An organism described . as_______________, . YongxianCohen www.sciencedirect.comOpinionChemicalBiology observed Applications. UNIT. TOPIC. FACULTY. Unit I. Microbial biotechnology . Genetic manipulation, Engineering microbes for the production of antibiotics, enzymes, insulin, growth hormones, monoclonal antibodies, microbes for clearing oil spills. Introduction. Examples of Transgenic Animals. Finite Cell Lines. 1. 3. Creating Transgenic Animals. 2. Few Questions????. What is a Transgenic Organism. ?. A transgenic organism is one which contains novel heritable genetic material. 1. 2. 3. 4. Ti Plasmid & T-DNA transfer. . Herbicide . Resistant . Insect Resistant . Drought/Stress resistant. Delayed . ripening. 5. Gene transfer in plants. Viral . transduction. Major Four.