PPT-Transgenic

Plants Genetically modified organisms plants Genetically engineered plants Why create transgenic plants When there is no naturally occurring genetic variation for the target trait  . 286 Transgenic Plants Methods and Protocols Edited by L Pe57585a 57513 Humana Press Inc Totowa NJ Stable Transformation of Plant Cells by Particle BombardmentBiolistics Julie R Kikkert Jos57577 R Vidal and Bruce I Reisch Summary Particle bombardment Plants. (Genetically . modified organisms (plants). Genetically engineered . plants) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . Christina Hill . Department of Biological Sciences, York College of Pennsylvania. Project Summary. Bovine Spongiform Encephalopathy (BSE) and Natural Scrapie are prion diseases that result from the mis-folding of proteins in the brain. One mis-folded protein can cause other proteins to mis-fold creating florid plaques that can affect motor function and decrease lifespan. Prion diseases can be transferred to animal species that are not naturally susceptible to these diseases through gene transfer. Research shows that brain plaque isolates from animals infected with prion diseases can be collected and inserted into the brains of transgenic mice. In the proposed experiment, 10 (OvTgPrP4) transgenic mice will be injected with normal saline, 10 with BSE isolates and 10 with Natural Scrapie isolates. Inoculated transgenic mice are observed for changes in motor function each week for course of their lifespan. After expiration, the mice can be dissected and their brain removed. The removed brains would be paraffin-embedded for microtome sectioning. These sections will be stained using Immunohistochemistry to look for the presence of florid plaques associated with the respective disease. Isolates from each mouse brain will also be tested for infection using a Sandwich ELISA test. Infection in all mice inoculated with the respective diseases should be found, as well as a decrease in motor dysfunction and lifespan. Effective transmission of prion diseases to species without natural susceptibility can allow for more effective medical models to be developed and increased knowledge of these diseases.. The Importance of Evolution in Ecology. Ecologists study proximate or “how” questions:. How nutrients flow through ecosystems. Evolutionary biologists study ultimate or “why” questions:. Why does a stickleback fish have armor in one lake but not another?. 1.) . Selective breeding . = The process by which desired traits of certain plants and animals are selected and passed on to their future generations.. Breed only those plants or animals with desirable traits. S Dasari. 1. ,. . S Kesh. 1. , J Buursma. 2. , S Livingston. 2. , D Kearn. 2. ,B Herndon. 1. , G . Vanden. Heuvel. 3. , R Baybutt. 2. , A Molteni. 1. .. 1. UMKC School of Medicine, . 2. Wheaton College, . chromosome. Foreign DNA. cytoplasm. nucleus. Inheritance. Mendalian. or . non-. mendalian. : . non-. mendalian. manner of inheritance is indicative of . transgene. instability. Faithful expression or . Dr. Kamal Kumar Gupta. Mice from Dr. Ralph L. . Brinster's. Giant . M. ouse Experiment . T. he human . growth hormone gene was expressed in the liver of mice. Knockout Mouse. A . mouse in which a gene affecting hair growth has been knocked . KAMAL KUMAR GUPTA. Transgenic Animals. Dolly. What is a transgenic animal? . A transgenic animal is one whose genome has been changed to carry genes from other . species. transgenes are functionally expressed and stably inherited in the transgenic animals.. modified organisms (plants). Genetically engineered . plants)) . Why create transgenic plants? . When there is no naturally occurring genetic variation for the target trait..  . Examples: . Glyphosate herbicide resistance in soybean, corn . fragmentDetermining Transgene Copy Number and Number ofIntegration Sites in Transgenic MiceThom Saunders. University of Michigan Transgenic Core. http://www.med.umich.edu/tamc Assumption: the Haploid Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 October 2022 Downloaded from http://rupress.org/jem/article-pdf/176/4/991/1103092/991.pdf by guest on 28 Oct Introduction. Examples of Transgenic Animals. Finite Cell Lines. 1. 3. Creating Transgenic Animals. 2. Few Questions????. What is a Transgenic Organism. ?. A transgenic organism is one which contains novel heritable genetic material. 1. 2. 3. 4. Ti Plasmid & T-DNA transfer. . Herbicide . Resistant . Insect Resistant . Drought/Stress resistant. Delayed . ripening. 5. Gene transfer in plants. Viral . transduction. Major Four.