Nonessential Amino Acid Metabolism in Healthy Adult Males PowerPoint Presentation

Nonessential Amino Acid Metabolism in Healthy Adult Males PowerPoint Presentation

2017-10-21 57K 57 0 0

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Leah . Cooper. Department . of Pediatrics, University of British Columbia & Child and Family Research Institute, BC Children's . Hospital. Outline. Background. Protein. Amino . Acids. Indicator Amino Acid Oxidation Technique. ID: 598089

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Presentations text content in Nonessential Amino Acid Metabolism in Healthy Adult Males

Slide1

Nonessential Amino Acid Metabolism in Healthy Adult Males

Leah CooperDepartment of Pediatrics, University of British Columbia & Child and Family Research Institute, BC Children's Hospital

Slide2

Outline

Background

Protein

Amino

Acids

Indicator Amino Acid Oxidation Technique

Objective & Hypothesis

Methods

3 Steps in Study

Results

Conclusion

Future

Directions

Slide3

Background - Protein

Structural

component of the bodyEnzymes, Hormones, Neurotransmitters, Transport MoleculesMade of 20 Amino AcidsNeed all 20 in correct proportions for protein synthesis to occur in the body

www.onlymyhealth.com

Slide4

Background – Amino Acids

www.thehealthsite.com

www.rpi.edu

Slide5

Background - Evolution

http://

www.nature.com

/

scitable

/

topicpage

/an-evolutionary-perspective-on-amino-acids-14568445

Slide6

Background - Nitrogen

Play many metabolic roles in the body

The requirement for

total

nitrogen

is the primary determinant of total protein requirement

If total amino nitrogen is inadequate, synthesis of nonessential amino acids and thus protein is less than maximal

Slide7

Some nonessential amino acids (NEAA) may supply nitrogen better than othersRose et al. (1948) showed increased growth by 70-75% in rats fed essential amino acids (EAA) + NEAARechcigl et al. (1957) showed different growth rates in ratsGlutamate gave the largest increase in growthFollowed by Alanine, Aspartate, Asparagine, Proline, and GlutamineSmallest increases in growth were Glycine and Serine

Background – Nitrogen Supply

Slide8

Background - Indicator Amino Acid Oxidation Technique

Previous Methods (N balance) were long in duration, very invasive and impractical

The stable isotope based IAAO is rapid, minimally invasive and accurate

IAAO measures the

oxidation of

the tracer

13

C-PHE to

13

CO

2

in breath

O

xidation

is inversely proportional to protein

synthesis

High Oxidation = Low Protein Synthesis

Slide9

Objective:

To

determine the metabolic demand

of

9 of the

NEAA

using

the indicator amino acid oxidation (IAAO) method in healthy adult

males

Hypothesis:

There will be

a

hierarchy

among the 9 NEAA,

more specifically

Alanine, Arginine, Asparagine, Aspartate, Glutamine, Glutamate, Glycine,

Proline

,

and

Serine

,

to supply Nitrogen to the body for the de novo synthesis of the other NEAA and for protein

Slide10

Methods

Slide11

Preliminary Study

Target Population: Healthy adult males Between the ages 20-40classified as normal body weight and BMI (18.5-25 kg/m2)Background Information: Medical & Dietary historiesAnthropometric measurementsBody composition Resting energy expenditure (REE) Informed consent

Slide12

Methods

Slide13

2 days prior to each study dayAdaptation diets contain a protein intake of 1.0 g/kg and a caloric intake of 1.7 × REE Provided as 6 milkshakes.

Adaptation

products.nestle.ca

Slide14

Methods

Slide15

10 Study DaysRandomizedDiet: The Base Diet (0.34 g/kg/day) Only EAAThe Base Diet + 1 NEAA (0.73 g/kg/day) 50:50 ratio of EAA: NEAA 5th Meal starts the isotope tracer protocol with an oral dose of L-1-13C- Phenylalanine

Study Day Diet

Base + ALA

BASE (EAA)

Base + SER

Week 1

Week 2

Base + GLN

BASE + PRO

Base + ARG

Week 4

Week 5

Etc…for 10 weeks

Study Day 1 Study Day 2 Study Day 3

Study Day 4 Study Day 5 Study Day 6

Slide16

Study Day Sampling

Sample Collection

Breath and Urine

Baseline

Isotopic Steady State

Carbon dioxide production (VCO2)

Slide17

13CO2 oxidation values analyzed using repeated measures ANOVA (Graphpad PRISM)

Sample Analysis & Statistics

Multi-flow

Continuous Isotope Ratio Mass Spectrometer

Slide18

Results – 50 Study Days

Slide19

The first study to look at NEAA in humansWe suggest all NEAA can satisfy the metabolic demand for total nitrogen.

Conclusion

Slide20

Determine whether a difference in NEAA metabolic demand exists in more vulnerable populations

Future Directions

www.vancitychiro.com

Slide21

Acknowledgments

Dr. Rajavel ElangoLab membersAjinomoto Inc. Co.Child and Family Research InstituteUniversity of British ColumbiaAll my participants!

Slide22

Rose, W.C., Oesterling, M.J., and Womack, M. (1948). Comparative growth on diets containing ten and nineteen amino acids with further observations upon the role of glutamic and aspartic acids. J Biol Chem 176: 753-762.Rechcigl Jr., M., Loosli, J. K., Williams, H. H. (1957). The net utilization of non-specific nitrogen sources for the synthesis of non-essential amino acids I. Growth and nitrogen utilization. J Nutr 63: 177-192.Millward DJ. Methodological considerations. Proc Nutr Soc 2001;60: 3–5. Food and Agriculture Organization of the United Nations. Energy and Protein Requirements (1991).

References

Slide23

QUESTIONS ??

Thank you for listening !!

Slide24

Study Diet

Study Day Base Diet

Slide25


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