Department of Biology and Department of Bioengineering The University of Pennsylvania Philadelphia PAAuthors for correspondence email zhqisasupennedu markjoseasupennedu Hunting for the ID: 960768
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Huntingtons disease (HD) is a devastatingneurodegenerative disorder, afflicting threeto ten individuals per 100,000 in WesternEurope and North America (Gil and Rego,2008). In 1993, the gene responsible for HDHTT)was cloned, representing a majorbreakthrough in the field. HTTencodes alarge protein that was named Huntingtin(Htt). The N-terminal portion of Htt con-tains a stretch of glutamines (the polyQtract) and HD patients harbor pathogenicpolyQ expansions (Andrew et al., 1993;Bates et al., 1998). Although the polyQlength can vary, all healthy individuals havefewer than 37 glutamines and those withgreater than 40 are certain to develop HD(Rubinsztein et al., 1996). Moreover, thelength of the polyQ expansion correlatesinversely with the age of disease onset(Andrew et al., 1993). Experiments in cell Department of Biology and Department of Bioengineering, The University of Pennsylvania, Philadelphia, PA*Authors for correspondence (e-mail: zhqi@sas.upenn.edu; markjo@seas.upenn.edu) Hunting for the function ofHuntingtin JOURNAL CLUB DMM Disease Models & Mechanisms additional parameters to more clearly definethe role of dHtt in Drosophila. Geneticmodifier screens will probably identifygenes and pathways that interact with dhttto expand our understanding of dHtt func-tion and HD pathogenesis. Also, becausethe HD-Q93;dhtt-komodel mimics bothgain- and loss-of-function aspects of thedisease, this may be a useful systemforiden-tifying andtestingcandidate compoundsthat could ameliorate HD pathogenesis [e.g.histone deacetylase inhibitors (Steffan et al.,2001)].Thanks to the simplicity and ele-gance of the fly, we may soon step closer tounderstanding the normal and pathogenicfunctionsof Huntingtinin humans.Andrew, S. E., Goldberg, Y. P., Kremer, B., Telenius, H.,Theilmann, J., Adam, S., Starr, E., Squitieri, F., Lin,B., Kalchman, M. A. et al.(1993).The relationshipbetween trinucleotide (CAG) repeat length andclinical features of Huntingtons disease. Bates, G. P., Mangiarini, L., Wanker, E. E. and Davies,S. W.(1998).Polyglutamine expansion andHuntingtons disease. Biochem. Soc. Trans.Bilen, J. and Bonini, N. M.(2005).Drosophila as amodel for human neurodegenerative disease. Annu.Rev. Genet.Cattaneo, E., Rigamonti, D., Goffredo, D., Zuccato, C.,Squitieri, F. and Sipione, S.(2001).Loss of normalhuntingtin function: new developments inHuntingtons disease research. Trends Neurosci.Duyao, M. P., Auerbach, A. B., Ryan, A., Persichetti, F.,Barnes, G. T., McNeil, S. M., Ge, P., Vonsattel, J. P.,Gusella, J. F., Joyner, A. L. et al.(1995).Inactivationof the mouse Huntingtons disease gene homologScienceGil, J. M. and Rego, A. C.(
2008).Mechanisms ofneurodegeneration in Huntingtons disease. Eur. J.Neurosci.Gunawardena, S., Her, L. S., Brusch, R. G., Laymon, R.A., Niesman, I. R., Gordesky-Gold, B., Sintasath, L.,Bonini, N. M. and Goldstein, L. S.(2003).Disruptionof axonal transport by loss of huntingtin or expressionof pathogenic polyQ proteins in Drosophila. NeuronImarisio, S., Carmichael, J., Korolchuk, V., Chen, C. W.,Saiki, S., Rose, C., Krishna, G., Davies, J. E., Ttofi, E.,Underwood, B. R. et al.(2008).Huntingtons disease:from pathology and genetics to potential therapies.Jackson, G. R., Salecker, I., Dong, X., Yao, X., Arnheim,N., Faber, P. W., MacDonald, M. E. and Zipursky, S.(1998).Polyglutamine-expanded human huntingtintransgenes induce degeneration of Drosophilaphotoreceptor neurons. NeuronLi, Z., Karlovich, C. A., Fish, M. P., Scott, M. P. andMyers, R. M.(1999).A putative Drosophila homologof the Huntingtons disease gene. Hum. Mol. Genet.Nasir, J., Floresco, S. B., OKusky, J. R., Diewert, V. M.,Richman, J. M., Zeisler, J., Borowski, A., Marth, J. D.,Phillips, A. G. and Hayden, M. R.(1995).Targeteddisruption of the Huntingtons disease gene results inembryonic lethality and behavioral andmorphological changes in heterozygotes. CellRubinsztein, D. C., Leggo, J., Coles, R., Almqvist, E.,Biancalana, V., Cassiman, J. J., Chotai, K., Connarty,M., Crauford, D., Curtis, A. et al.(1996).Phenotypiccharacterization of individuals with 30-40 CAG repeatsin the Huntington disease (HD) gene reveals HD caseswith 36 repeats and apparently normal elderlyindividuals with 36-39 repeats. Am. J. Hum. Genet.Steffan, J. S., Bodai, L., Pallos, J., Poelman, M.,McCampbell, A., Apostol, B. L., Kazantsev, A.,Schmidt, E., Zhu, Y. Z., Greenwald, M. et al.Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila. NatureZeitlin, S., Liu, J. P., Chapman, D. L., Papaioannou, V.E. and Efstratiadis, A.(1995).Increased apoptosisand early embryonic lethality in mice nullizygous forthe Huntingtons disease gene homologue. , S., Feany, M. B., Saraswati, S., Littleton, J. T.and Perrimon, N.(2009). Inactivation of DrosophilaHuntingtin affects long-term adult functioning andthe pathogenesis of a Huntingtons disease model.Dis. Model. Mech.dmm.biologists.org JOURNAL CLUB Journal Club articles discuss important discoveries in model organism research and are authored by graduate/medical students,postdocs or clinical fellows. Articles presented should be published in or other peer-reviewed research journals. If youhave an idea for Journal Club or are interested in writing, contact the editorial staff at dmmreviews@biologists.com.