PDF-IHC antigen retrieval

protocol H eat induced epitope retrieval and enzymatic retrieval 2 IHC antigen retrieval protocol Introduction Most formalin fixed tissues require an antigen retrieval step before immunohistochem. Group . 3. :. M. . Yusron. . Hasani. . (. 105090100111037. ). A. Muammar . Kadafi. . (115090101111012. ). Nira. . Meirit. a. W.. . . (. 115090100111017. ). Putri. . Indisari. . (. 1150901. INST 734. Module 3. Doug . Oard. Agenda. Ranked retrieval. Similarity-based ranking. Probability-based ranking. Boolean Retrieval. Strong points. Accurate, . if you know the right strategies. Efficient for the computer. reactions. Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . (for MODIS). Andy Harris. Jonathan . Mittaz. Prabhat. . Koner. (Chris Merchant, Pierre . LeBorgne. ). Satellite data – pros and cons. Main advantages of satellite data. Frequent and regular global coverage (cloud cover permitting for IR). Information Retrieval. Information Retrieval. Konsep. . dasar. . dari. IR . adalah. . pengukuran. . kesamaan. sebuah. . perbandingan. . antara. . dua. . dokumen. , . mengukur. . sebearapa. . Let’s start. The immune system is a system of biological structures and processes within an organism that protects against disease.. . To function properly, an immune system must detect a wide variety of agents, from viruses to parasitic worms, and distinguish them from the organism’s own healthy tissue. . Hongning. Wang. CS@UVa. What is information retrieval?. CS6501: Information Retrieval. CS@UVa. 2. Why information retrieval . Information overload. “. It refers to the . difficulty. a person can have understanding an issue and making decisions that can be caused by the presence of . Fatemeh. Azimzadeh. Books. (Manning et al., 2008). Christopher D. Manning, . Prabhakar. . Raghavan. , and . Hinrich. . Schütze. . Introduction to Information Retrieval. Cambridge University Press, 2008. . By the end of this session student should be able to know. . Definition of antigen. . Factors influencing immunogenicity. Biological classes of antigens. . ANTIGEN. Defined as any substance that satisfies two distinct immunologic . Dr. Suzan Y.. Antigen. :. It s a substance that may be specifically bound by an antibody (. Ab. ) molecule or T cell receptors (TCR). When this binding lead to the activation of B cells or T cells ,this substance is known as . ELISA. Immunofluorescence. Flow cytometry. Western blotting. ELISA. We use an antibody that’s bound covalently to an enzyme to bind:. -antigen (direct ELISA). -antibody that is bound to antigen (indirect ELISA). . Presentation. MHC-restricted antigen . recognition by . T. . cells. Any . T . cell can . recognize . an . antigen . on an APC only if . that antigen . is . displayed . by MHC. . molecules. Antigen . Presenting Cells 99 attention has been paid to the APC require- ments for class II-restricted activation of T helper/inducer cells than class I- or class II- restricted cytotoxic T cells. Thus, most Detection By. Precipitation Methods. Dr.T.V.Rao MD. 11/11/2014. Dr.T.V.Rao MD. 1. Beginning of Serology. Serology as a science began in 1901. Austrian American immunologist . Karl Landsteiner. (1868-1943) identified groups of red blood cells as A, B, and O. From that discovery came the recognition that cells of all types, including blood cells, cells of the body, and microorganisms carry proteins and other molecules on their surface that are recognized by cells of the .