PPT-Searching for Transcription Start Sites in

Drosophila 062016 Wilson Leung Outline Transcription start s ites TSS annotation goals Promoter architecture in D melanogaster Find the initial transcribed exon Annotate putative transcription start sites. Tim . Johnstone. BIOL1220. Spring 2010. Transcription Factor. a protein that binds to specific DNA . sequences to modulate the transcription of DNA to mRNA. The transcription factor TATA binding protein (blue) bound to DNA (red). Image by David S. . Central Dogma of Biology. Information flows from . DNA .  RNA  Proteins. Think of it as exchanging money in a different country: proteins cannot be built directly from DNA. Three main processes involved:. DNA. RNA. PROTEIN. RNA and Transcription. RNA. R. ibo. n. ucleic . A. cid. another type of nucleic acid similar to DNA found in cells. also a polymer made of nucleotides. takes information from the nucleus to the rest of the cell. C483 Spring 2013. 1. RNA Polymerase. Polymerizes RNA in a 3’. 5’ direction.. Has 3’5’ . exonuclease. activity. Has 5’3’ . exonuclease. activity. Catalyzes a reaction driven by pyrophosphate hydrolysis. C483 Spring 2013. 1. Which . of the following is true about . transcription regulation? . A) A repressor protein activates transcription of a negatively regulated gene. . B) A positively regulated gene can be transcribed only in the absence of active repressor. . RNA Processing, and Transcriptional Regulation. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. Geeta. . Narlikar. ) . . January 19, 2017 – Transcription Initiation and its Regulation in Bacteria. January 23, 2017 – Transcription Initiation and its Regulation in Eukaryotes. January 26, 2016 – Chromatin 1. BNFO 602/691. Biological Sequence Analysis. Mark Reimers, VIPBG. Why TFBS?. Protein transcription factors were among the first gene regulators identified. Many TFBS have distinct sequences, which were suitable for bioinformatics analysis. Topic 2.6 & 7.2. Understandings:. Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase. . Gene expression is regulated by proteins that bind to specific base sequences in DNA.. Structure. Central Dogma. Template Strand. Coding Strand. RNA Functions. RNA Building Blocks. Structure. Eukaryotic Process. RNA Polymerase. Subunits (Prokaryotes). α (2). β. β’. ω. σ. Transcription. synthesis). A. Prokaryotes: RNA polymerase, . Promoters – sigma factor. B. Eukaryotes: RNA polymerases, . Promoters – transcription factors. . 1 strand of . dsDNA. used as template to synthesize complimentary . Drosophila. 01/2019. Wilson Leung. Outline. Transcription start sites (TSS) annotation goals. Promoter architecture in . D. melanogaster. Find the initial transcribed exon in the target species. Annotate putative transcription start sites. . SYFTET. Göteborgs universitet ska skapa en modern, lättanvänd och . effektiv webbmiljö med fokus på användarnas förväntningar.. 1. ETT UNIVERSITET – EN GEMENSAM WEBB. Innehåll som är intressant för de prioriterade målgrupperna samlas på ett ställe till exempel:. Chargaff's rule. states DNA of any species contains equal amounts of guanine and cytosine and also equal amounts of adenine and thymine. . This pattern is found in both strands of the DNA. . They were discovered by Austrian chemist Erwin Chargaff.