PPT-Refinement against cryo

EM maps Garib Murshudov MRCLMB Cambridge UK Contents Importance of phases Fit into EM maps likelihood and restraints Overfitting Effect of oversharpening Form factors Brown et al 2015 Tools for macromolecular model building and refinement into electron . Fluidity of Two Commercial DAHLE, P.A. TONDEL, C.J. PARADIES, and L. ARNBERG The effect of grain refinement on the fluidity of A1Si7Mg and A1Sil 1Mg has been investigated by spiral tests. Two diffe Maximize agreement with diffraction data. Minimize R-factor. Maximize ideality of stereochemistry. Minimize deviation from ideal bond lengths and angles. S. |F. obs. -F. calc. |. S. |F. obs. |. hkl. hkl. Swanand. Gore & Gerard . Kleywegt. May 6. th. 2010, 12-1 pm. Macromolecular Crystallography Course. Outline. Intuitive idea of resolution – why higher order diffraction is better.. Parameters, model, observations, refinement – more data is better.. We cannot complete that refinement due to a little problem: in order to get the new values and , we need not only the values of and just produced by the recursive call, , which was not sav Inference. Hiroshi Unno (University of Tsukuba). Joint work with: Naoki Kobayashi, . Tachio. Terauchi, . Ryosuke. Sato, Takuya . Kuwahara. , . Kodai. Hashimoto, . Sho. Torii. 2015/7/4. HOPA 2015. Eric Roberts. CS 106A. January 8, 2016. Outline. Review Karel statement forms. 1.. Exercise: The . putBeeperLine. method. 2.. Stepwise refinement. 3.. Exercise: The . BanishWinter. program. 4.. Preconditions and postconditions. procedure for . native structure. phenix.refine. . yourcoords.pdb. . m230d_2017_scaled.mtz . refinement.input.xray_data.labels. =". FP_native-cris. . SIGFP_native-cris. “ . output.prefix. =. nativeround1. Toll-Free (US & Canada):. 866.740.1260 . Toll:. 303.248.0285 . Access Code:. . 8304505. 1920. ReadyTalk. . link. . https://core.readytalk.com/interface/guest.jsp?host=readytalk&an=8667401260&ac=8304505. AdV- VAC CTRL SYS/Cryo Interface 06Feb2013 Cryotrap cryogenics (simplified sketch) = the ‘electronic’ equipment needed to read LN2 level, temperature, controller of feeding valve, phase separator electronics... available. Network . connections: added 4 managed switches.. M7 has slow controls . added. Cryo. , vacuum, ODH information . available. Vacuum . gauges reading through . Moxa. box and Java device server and put in Timber.. Enough space to organize between the cryostats an effective clean room (including personal SAS) to prepare APAs and CPAs to be installed. Enough space to be able to bring in the cryostats the material for the cold vessels. 08: 50 - 09 :00 Opening : Zihe Rao 09: 00 - 09 :50 Chairman: Zihe Rao Jack Johnson, The Scripps Research Institute Title: Biophysical Studies of Virus Particles and their Maturation: insights 0850 -0900 OpeningZiheRao0900 -0950Chairman ZiheRaoJack Johnson The Scripps Research InstituteTitle Biophysical Studies of Virus Particles and their Maturation insights into elegantly programmed na Randy Kimble. 1. Pamela Davila. 1. , Charles Diaz. 1. , Lee Feinberg. 1. , Stuart Glazer. 1. , Gregory Jones. 2. , Rob Luetgens. 2. James Marsh. 1. , Gary Matthews. 3. , Douglas McGuffey. 1. , Pat O’Rear.