PPT-From Smith-Waterman to BLAST

Jeremy Buhler in absentia Wilson Leung 072015 Key limitations of the Smith Waterman local alignment algorithm Quadratic in time and space complexity Report only one optimal alignment. Basic Local Alignment Search Tool. (Altschul et al. 1990. ). What is blast. ?. BLAST .  DNA/protein databases. GOOGLE  internet. . D. Fristrom, Boston U. .. Uses for Blast. . Identifying unknown sequence. Surg Cdr Jane Risdall . Royal Navy. Consultant. . Neuroanaesthetist/Intensivist. Senior Lecturer, Academic Dept Military . Anaesthesia. and Critical Care. Visiting Research Fellow, University of Cambridge. Yanbin Yin. Fall 2014. 1. http://. www.ncbi.nlm.nih.gov. /books/NBK52640/. 2. Why you do need to run BLAST in command line terminal?. NCBI’s server does not have a database that you want to search. 1914 and 1915, Wyndham Lewis, ed.. Percival Wyndham Lewis. 1882-1957. the men of 1914. unpopular. politics – questionable at best. artist, writer, thinker. The Enemy – and its pitfalls. Vorticism. et BLAST . avanc. é. J.. S. .. Bernardes. /H. . Richard. Mat. ériel. :. Bioinformatics and Functional Genomics. Jonathan Pevsner, . Wiley-Blackwell. ed.. Outline. Introduction. . Definition of . Yanbin Yin. Fall 2014. 1. http://. www.ncbi.nlm.nih.gov. /books/NBK52640/. 2. Why you do need to run BLAST in command line terminal?. NCBI’s server does not have a database that you want to search. 03/14/2017. 1. Leonardo . Mariño-Ramírez. NCBI, NIH – Bethesda, USA. NCBI Search Services and Tools. Entrez. . integrated literature and molecular databases. Viewers. BLink. . protein similarities. Recap : . Pairwise. sequence alignment. Grouping of two sequences to maximize similarity/identity using a scoring system (substitution matrices):. PAM (1,120,250). BLOSUM (80,62,45). Optimal alignment algorithms:. Adapted from College Board’s “Investigation 3: Comparing DNA Sequences to Understand Evolutionary Relationships with BLAST”. What we know so far.... We already know that DNA can be used to identify animal species at a genetic level. Thermo Fisher / Open Biosystems. Dr. Krishnan K. . Chittur. University of Alabama in Huntsville. Dr. Joseph Ng. University of Alabama in Huntsville. What is BLAST. B.L.A.S.T. stands for . B. asic . L. are what most people think of as rocket scientists. They research, design, and develop nearly anything that flies, such as airplanes, spacecraft, and of course rockets. . Blast Off . helps explore how different rocket designs change the ability of the rocket to go great distances. Follow the advice of the station helper to find out if a structure makes a difference and why so you can make the best rocket based on the distance it travels while maintaining a fairly straight path. . Jundi. Wang. School of Computing. CSC691. 09/08/2013. 2. Overview. 1.Introduction of BLAST . Background of BLAST. Programs in BLAST. Function of BLAST. 2.Application of BLAST. Outline I ntroduction BLAST s earch s teps Step 1: Specifying Sequence of interest Step 2: Selecting BLAST Program Step 3: Selecting a Database Step 4: Selecting Search Parameters and Format Team2. 邱冠儒 黃. 尹. 柔 田. 耕. 豪 蕭. 逸. 嫻 謝朝茂 . 莊閔. 傑. 2014/05/12. 1. BLAST. Basic local alignment search tool. Enable to compare query sequence of amino-acid or protein or DNA to database .