PPT-Topic – DNA Technology

Presented by Ms P H Giri Department of Microbiology Deogiri College Aurangabad BSc T Y Semester VI Paper No XIX Recombinant DNA Technology Ms Priyanka H Giri Steps in RDT. DNA: Picture 51. http://info.bio.cmu.edu/courses/03231/ProtStruc/ProtStruc.htm. B-DNA: The advent of modeling. http://molvis.sdsc.edu/pdb/dna_b_form.pdb. 46 Å. . 12 base sequence. (1953-2003). DNA: Size, Shape & Self Assembly. Unless you have an identical twin, you, like the sisters in this picture will share some, but not all . characteristics . with family members. DNA. The . instructions. for inherited traits are called genes.. DNA from a few Angles. Cell: . Every cell contains a nucleus which is filled with the directions for cell function, DNA. . Chromosome. : . When DNA is tightly coiled and wound it becomes visible in the form of . “DNA is a helical structure” . with “two co-axial molecules.”. “period is 34 . Å. ” . “one repeating unit contains ten . nucleotides on each of two . . . co-axial molecules.'’. “The phosphate groups lie on the outside of the structural unit, on a helix of diameter about 20 . shapes and patterns. 조연우. Contents. DNA. DNA origami. The application of DNA origami. DNA origami structure. “Normal DNA”. 이중나선 형태로 존재. Double Stranded, Double Helix. Adenine, Thymine, Guanine, Cytosine . Here are some examples that you may use: . Should school uniforms be enforced everywhere? . Should . men get paternity leave from work. ? . Are . we too dependent on computers. ?. . Should animals be used for research. 7. Lactose digestions in . E. coli. begins with its hydrolysis by the enzyme . -. galactosidase. , . lacZ. , is part of a . coordinatley. regulated operon containing the genes required for lactose utilization. Which of the following figures correctly depicts the interactions at the . Brief Chapter Outline.  . I. Cause for Concern? Recombinant DNA Technology: Promise and Controversy. Cutting . and Joining DNA. DNA . Cloning. V. Cloning Vectors. A. . Bacterial Vectors. Essential idea: . The structure of DNA allows efficient storage of genetic information. . 2.6 Structure of DNA and RNA. Nature . of science: . Using . models as representation of the real world—Crick and Watson used model making to discover the structure of DNA. (1.10) . The helical structure of . DNA. Formation of Nucleotides. Structure of polynucleotide polymer. Each base has its preferred . tautomeric. form. Purine. and . Pyrimidine. Base . tautomers. ; are frequent sources of errors during DNA synthesis.  . Author: Michael Fenech. Affiliation: Genome Health Foundation, North Brighton, SA, 5048, Australia. Email: . mf.ghf@outlook.com. . Introduction. Life as we know it depends entirely on the capacity of cells to utilize energy and molecules in the environment for cellular function and reproduction. . In . their 1953 announcement of a double helix structure for DNA, Watson and Crick stated, . "It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.". Structure of. DNA and RNA. DNA Replication. Transcription & Translation. Nucleotides. 3 major nucleic acids:. DNA, RNA, ATP. DNA & RNA. Made of nucleotides (monomer). Nucleotides:. One phosphate. Topic 4.4 –guide. STATE.  that, in gel electrophoresis, fragments of DNA move in an electric field and are separated according to their size.. STATE.  that gel electrophoresis of DNA is used in DNA profiling..