PPT-Primer Design & Restriction Analysis

3 rd December 2014 Carrie Iwema PhD MLS AHIP Information Specialist in Molecular Biology Health Sciences Library System University of Pittsburgh iwemapittedu httpwwwhslspittedumolbio . PCR provides a forensics tool for identifying colonies. Three strains look alike!. How can you identify the strains?. Geneticists like to verify their strains’ genotypes before experiments. Photo by Yellowstone NPS. An Introduction to Restriction Enzymes & Gel Electrophoresis. Objectives. Understand the use of restriction enzymes as biotechnology tools. Become familiar with principles and techniques of . agarose. Daniel Svozil. Software choice. source: Bioinformatics for Dummies. Dotlet. . Learn by example – use the sequence from the Repeated domains. In . this case, the darker the pixel, the lower the . score.. Using Restriction Enzymes. Learning targets for Restriction . Digestion and Analysis of Lambda DNA . lab?. I will understand . the use of restriction enzymes as biotechnology . tools. I will become . Site-specific restriction endonucleases are used to identify DNA molecules. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . Vocabulary. Karyotype. Autosome. Sex chromosome. Nondisjunction. Monosomy. Trisomy. Pedigree. Carrier. Restriction enzyme. Restriction site. Restriction fragment. Sticky ends. Recombination. Gel electrophoresis. factors . increase. in response . to. . viral. rebound . after. . analytical. treatment . interruption. . De Scheerder Marie-Angélique, Van Hecke . Clarissa. , De . Langhe. Nele, Sips Magdalena, . Objectives. -Understand how Restriction Enzymes digest DNA.. -Know how to construct a . pAMP. . (plasmid) or gel.. -Given the size of fragments, gel, know how to construct a restriction map.. -Given a restriction map know how to construct a gel.. What are restriction endonucleases (REs)?. How can REs be used to identify DNA molecules?. How can I find RE recognition sites in the . MET. plasmids? . Restriction endonucleases are part of a bacterium’s defense against invaders. Primer Design Molecular cloning requires manipulation of nucleic acid, including copying (synthesize), cutting (digest) and pasting (ligate). In bacteria, it is usually done in plasmid form. To inse reproduce modified versions of Figures 4-3, 11-12, 12-3 (a), 12-6, 12-7 (c), 12-7 (d), 14-20, 14-24, 14-29 and 17-10 (a) from the book published by them in 1996 with the titleIntroduction to Genetic A reproduce modified versions of Figures 4-3, 11-12, 12-3 (a), 12-6, 12-7 (c), 12-7 (d), 14-20, 14-24, 14-29 and 17-10 (a) from the book published by them in 1996 with the titleIntroduction to Genetic A the isolation of the restriction . endonuclease. from. E. coli K (. Meselson. & Yuan 1968). . It was evident . that the restriction . endonucleases. from . E. coli K . and . E. coli B were important examples of proteins. Choi Y, Jang W, Kim J, Ryu J, Lee S, Park K, et al. Spotted Fever Group and Typhus Group Rickettsioses in Humans, South Korea. Emerg Infect Dis. 2005;11(2):237-244. https://doi.org/10.3201/eid1102.040603.