PDF-13.T.-C.Heetal.Proc.Natl.Acad.Sci.U.S.A.,250914.W.Bains,P.Ponte,H.M.Bl

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CraniofacialDevelopmentalBiologyandRegenerationBranchNationalInstituteofDentalandCraniofacialResearchNationalInstitutesofHealthBethesdaMD20892USATowhomcorrespondenceshouldbeaddressedEmailky4

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13.T.-C.Heetal.Proc.Natl.Acad.Sci.U.S.A.,250914.W.Bains,P.Ponte,H.M.Bl: Transcript

CraniofacialDevelopmentalBiologyandRegenerationBranchNationalInstituteofDentalandCraniofacialResearchNationalInstitutesofHealthBethesdaMD20892USATowhomcorrespondenceshouldbeaddressedEmailky4. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA74(1977)4133.(iii)abFIG.5.Thepackingoftwoindependentpleatedsheets.(a)Twopleatedsheetsarerepresentedbytwothinsmoothsheets.Arrowsindicatethechaindirection.Eachsheethasaright-handed Proc.Natl.Acad.Sci.USA90(1993)10391days.Whenthecellswereforamicroinjectionexperiment,coverslipswerefirstmarkedwithadiamond-tippentofacilitatethelocalizationandvisualizationofmicroinjectedcells.After8- Proc.Natl.Acad.Sci.USA90(1993)RESULTSElectronMicroscopyofPNA-DNAComplex.Weuseda(dA)98-(dT)98targetcontainedwithinasupercoiledplasmid,pA98,linearizedwiththeCfrlOIrestrictionenzyme,andchallengedthiswith Proc.Natl.Acad.Sci.USA83(1986)3195U3R[U311PUvir5DNALTR-RUSU+ViralRNABamHIXhoILTRydLTPrototypePIli3400(3200)NheINheILEZ3EIILIZ3~MTZIJL*3200NT0,2200NT-SVNEO*3000NT*2200NTMT-NBLTRdLTRSV-N"1C7272U3NheIPvu Proc.Natl.Acad.Sci.USA90(1993)11479knownconcentration.DNAwasstoredin1mMTris,pH7.8/0.1mMEDTA.Forinjections,DNAwasdilutedinsaline(0.9%NaCl).VaccineTrials.Vaccinetrialsinmicewereaccomplishedbytheadminist Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA92(1995)7141FRETefficiencyRNAbulgeA7AgFIG.2.KinkingofDNAandRNAduplexesbybasebulgesisrevealedbyFRET.Thedistancebetweendonor(D)andacceptor(A)fluorophoresattachedtothe5'terminiofthe Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag

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