dead fish i love you but youre bringing me down b acterial bioluminescence Is interesting Diverse Vibrios in the sea Goal to isolate bioluminescent bacteria for further study strategy ID: 476574
Download Presentation The PPT/PDF document "culturing bioluminescent bacteria" is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
Slide1
culturing bioluminescent bacteria
dead fish,
i
love you,
but you’re bringing me downSlide2
bacterial bioluminescence
Is interesting
Diverse
Vibrios in the seaGoal: to isolate bioluminescent bacteria for further studySlide3
strategy
Bioluminescent bacteria are present throughout marine environments
Bioluminescence is detectable when cells are present at high density
Therefore: make bacteria grow to induce luminescence, pick them outSlide4
Seawater complete mediaArtificial seawater,
bacto
tryptone, yeast extract, glycerol, agarSlide5
Photo courtesy S. RussellSlide6
How do we improve yields?
Sample from enriched locations
Improve
culturability (great plate count anomaly)In situ culturing with diffusion chambers?
D’Onofrio et al (2010) Chemistry & Biology. 17(3):256-264Slide7
How to improve yields? (cont’d)
More selective media, or bait
?
chemotaxisNon-symbiosis-related functions of bioluminescence?Improve detection on plateSlide8
the end