ACTIVITY OF CONSORTIUM INSTM - PowerPoint Presentation

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ACTIVITY OF CONSORTIUM INSTM

Apr. 1, 2015 – Oct. 31, 2015

Technical

Meeting

05-11-

2015

- Sergio Miele, Enrica Bargiacchi –

EU

project

LIFE13

ENV IT 000461 / “EVERGREEN”

“Environmentally friendly biomolecules from

agricultural wastes as substitutes of pesticides

for plant diseases control”Slide2

INSTM

Evergreen:

Targets and activities

Demonstrating the

in vitro

and

in vivo

efficacy and reliability of

polyphenolic

-based

biomolecules

extracted

from agricultural non-food biomass

and

fresh vegetable waste from ready-to-eat produce processors

as plant

biostimulants

and disease control products against

phytopathogenic

bacteria and nematodes,

to replace current agrochemicals

, included copper salts, in agriculture

Greater

sustainability

and

lower energy-intensive

crop production cycles

Lower

agrochemical

residue

hazard

in food and

pollution

in the environment, with less chance for resistance insurgence mechanisms

Reduction of point-source pollution

from disposal of vegetable waste in soil

Projecting new process of non-food biomass and fresh vegetable waste where these materials undergo to a polyphenol extraction step, and then are exploited to produce new materials, and energy (i.e. anaerobic digestion)

Sweet Chestnut (

Castanea

sativa

Mill.) is an example of a new process cycle to exploit biomass potentials, according to INSTM research Slide3

INSTM

Evergreen:

Castanea sativa

extracts

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3

4

5

6

7

8

9

WATER EXTRACTION

REVERSE OSMOSIS & NANO-FILTRATION

STANDARDIZED EXTRACTS

PRE-TREATMENTS

FEED FIBER (1% TANNINS)

MULCHING

TRACED

SOURCE

1

Courtesy: Gruppo Mauro SaviolaSlide4

INSTM

Evergreen:

Castanea sativa

extracts uses

1) Microgranulated starter fertilizers for application at planting/transplanting with

Castanea sativa

and other polyphenols extracts: a plant biostimulant for boosting plant earliness and use of soil nutrients and water

Courtesy:

Color Glass

S.p.A

.

2)

Castanea sativa

and other polyphenols extracts in fertigation: a plant biostimulant to increase plant resistance to nematodes and pathogens

Courtesy:

Fattoria

Autonoma

TabacchiSlide5

ACTIVITY FOR THE PROJECT Month 7-14

B1 / B7Demonstration of the performances of

traditional pesticides for the control of bacterial and nematode diseases of plants important for the

EU (2nd CYCLE EXPERIMENT-June/October 2015) Demonstration

of the in vivo performances of the high quality polyphenolic bioactive preparations, recovered from vegetable not edible biomass and waste, at pilot scale level in

field screenings (2nd CYCLE EXPERIMENT-June/October 2015) B4 - Demonstration

of the biological activity of the high quality polyphenolic extracts recovered from not edible biomass and waste, against plant pathogenic bacteria and nematode, in planta (Pot experiment in progress since Oct. 21, 2015)B7 – Preliminary activity: Microgranule formulations (April-May 2015)

C4 – Monitoring of the short term environmental benefits from the use of the high quality standardizes polyphenolic preparations in plant disease control at pilot scale level in field screenings:

Field soil sampling for residues

and nematodes (October 2015)

D7 - Articles and press releases

D10

-

International

conferences and

fairsSlide6

FIELD EXPERIMENT 2015: OXYMIL WAS UNAVAILABLE

the increasing trend toward a concentration of R&D for top used agrochemicals may represent a threat for world agriculture, because less a.i. and production sites are available, while pest resistance and accidental events can occur worldwide

;the necessity to develop alternative strategies for crop protection, less relying on bottle-neck agrochemicals, is therefore a must, to reduce market and food supply uncertainty.

problems in Jan. 2015 at the production site, the US manufacturer

DuPont’s La Porte factory near Houston, Texas no

Oxymil worldwideSlide7

Tobacco Virginia Bright, field grown, single plot 500 m2, June-Oct. 2015cv

PVH 2310 (low tolerance to Meloidogyne arenaria, and resistant to

Meloidogyne incognita race 1 and 3)Treatments

B1

–

Demonstration of the performances of traditional pesticides for the control of bacterial and

nematode diseases of plants important for the EU (2nd CYCLE EXPER.)Slide8

ConclusionsCHT: acts as a

biostimulant, with minimal effects on nematode count; low evidence of nematode feeding on tobacco roots was found. Better yield results for CHT, then the biological agent Flocter

, then agrochemicals

B1

–

Demonstration of the performances of traditional pesticides for the control of bacterial and nematode diseases of plants important for the EU (2nd CYCLE EXPER.)Slide9

B4 - Demonstration of the

biological activity of the high quality polyphenolic extracts recovered from not edible biomass and waste, against plant pathogenic bacteria and nematode, in

plantaTobacco Virginia Bright, greenhouse pot test, beginning Oct.21st

, 2015TreatmentsCHT Non-infested controlEtoprofos Infested controlCHT+Marc Meal (50%+50%)Slide10

B7 - Demonstration of the in vivo performances of the high quality poly-phenolic bioactive preparations

, recovered from vegetable not edible biomass and waste, at pilot scale level in field screenings (preliminary

activity: April-June 2015)

Preliminary activity consisted in preparing at industrial pilot level* formulations for field application of Chestnut tannins, to be used in 2015 growing season:

A microgranulated fertilizer for application at sowing/transplanting along the row (pilot plant)A fully and easily, non-foamy, wettable

tannin extract powder for application in microirrigation (pilot plant)A microgranulated composition of CHT + Marc Slide11

MAJOR MICROGRANULATED FERTILIZERS

WITH TANNIN EXTRACTS

%MICRO TAN

MICRO NPTannin extract18

12Nitrogen (N)07

Phosphate (P2O5)0

30Total Iron (Fe)32Complex Tannin-Fe (Fe)2.41.2Granule Size mm (> 80%) 100% 0.6-1.2 mm0.80.8

Authorization: Italian D.Lgs. 75/2010

as Iron-Tannin complex

fertilizer and Nitrogen-Phosphate Fertilizer

w/ Iron-Tannin complexSlide12

CORRECTIVES-VARIOUS CORRECTIVES *

DRY TANNINS EXTRACT WP

pHw

3.0 – 3.5ORGANIC CARBON (C)

MIN. 50 %TANNIN CONTENT

MIN. 73 %* Authorization: D.M. 30-06-2011, publ. on Italian

Official Gazette n° 215 Sept. 15, 2011 (modifications of D.Lgs. 75/2010)Slide13

INSTM ACTIVITY FOR THE PROJECT Month 7-13

D2 – Notice boards & information displayed on INSTM website (Link:

http://www.instm.it/0000000988.aspx), at the Chestnut Tannin production site, and at the Tobacco experiment site; D3 Demonstration workshop in Italy

 Visit to Tobacco experiments, and Ch Tannin microgranulate

fertilizer production site (July 17, 2015)D8 – Networking:

with Regione Umbria’s PSR funded Project QualiTaba (Traced quality of tobacco in a context of sustainable production in Central Italy)D10 - International conferences

and fairsPresentation at EXPO2015-Milano (June 25, 2015) of EVERGREENOral presentation at CORESTA Agro-Phyto Meeting, Izmir (Turkey), Oct. 25-29, 2015 (presentation on CORESTA WEB)D11 – Dissemination to institutions and policy makers Event at Accademia dei Georgofili

(Nov. 13, 2015)Slide14
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