PPT-Experiment 8 Separation of proteins by

ion exchange chromatography BCH 333practical Objectives 1 The objective of this experiment is for students to learn the principles of ion exchange chromatography by separating the charged molecules using a salt gradient. model. Wai. . Man . SZETO and Kin . Hong . WONG (KHWONG@CSE.CUHK.EDU.HK). The Chinese University of Hong Kong. DAFx-13, National University of Ireland, . Maynooth. , Ireland. Sep 2-5 2013. . 1. Faculty of Engineering, CUHK. Today only a small fraction of the energy in nuclear fuel is used. The spent fuel also has to be stored for about 100 000 years to be considered safe. The extremely long storage time is mainly due to the high radiotoxicity of the actinides. If, however, these long lived actinides could be separated from the spent fuel and transmuted, the energy efficiency would increase. In . An Anxiety Disorder. Anxiety Disorders . Separation Anxiety Disorder. Selective . Mutism. . Specific Phobia. Social Anxiety Disorder (social phobia). Panic Disorder . Agoraphobia. Generalized Anxiety Disorder . Steve Norton. The Centrosome. Microtubule organising centre during mitosis. One positioned either side of the mitotic spindle. Microtubules used to pull kinetochores apart – divide DNA between daughter cells. what is . homegeneous. ?. What is solute ?. Uniformly mixed – only see one layer. substance that dissolve in a solvent. What is solvent?. Is a substance that dissolves the solute. What is solution ?. Magnetic separation. Overview. Material is separated based on magnetic susceptibility. Highly magnetic material is removed using a hand magnet. Weakly magnetic materials are separated by a Frantz magnetic separator. Birth to Age 5. Information from:. Child Care Support Network. By: Rebecca Chopp. How Separation Anxiety Develops. Separation anxiety is a normal part of child development. Separation anxiety varies widely from child to child . Tutorial. lise schoonen. 14-12-’15. 1. What is gel electrophoresis?. Method for separation and analysis of macromolecules. DNA, RNA, proteins. Separation based on size and/or charge. Electric field. Chromatography. Chromatography is a technique of separation of different dissolved constituents of a mixture by absorbing them over an appropriate adsorbent material .It is a versatile method of separating different kinds of chemical mixtures .. 2. Proteins (. Polypeptides. ). Chains of Amino acids (. 20. different kinds). bonded together by . peptide bonds. . (. polypeptides. ). Made of . Nitrogen, Carbon, Oxygen, and Hydrogen. Functions:. Absorption and Stripping of Dilute Mixtures. In absorption (also called gas absorption, gas scrubbing, and gas washing), a gas mixture is contacted with a liquid (absorbent or solvent) to selectively dissolve one or more components by mass transfer from the gas to the liquid.. Most abundant molecule in cells making up 50% or more of their dry weight. Every protein has a unique structure, or shape. Allows it to perform a specific function. Enzymes are proteins that function as catalysts for many reactions in foods. Each amino acid (aa) shares a common structure; i.e. an amine (NH. 2. ) group, an acid group (COOH), and a central carbon atom bonded to hydrogen and to a side chain (R).. The side chains qualify aas as acidic, basic, neutral, aromatic, and sulphur-containing amino acids.. Presented by. Dr.K.ANBARASAN. Assistant professor and Head. Department of Botany . Padmavani. Arts and Science College for Women. Salem - 636 011. .. ELECTROPHORESIS. Electrophoresis is a molecular technique widely used in field of biochemistry, molecular biology and biotechnology to separate the bio molecules based on the migration of charged molecules..