PPT-Fixation and Fixative

Prepared by Reem Aldossari THE GENERAL VISCRA OF THE RAT Definition of Fixation In the fields of histology pathology and cell biology fixation is a chemical process by which . TOPIC:. FIXATION. SUBMITTED BY. RINSEENA M. GAFOOR. SUBMITTED TO. Mr. REJO JACOB JOSEPH. &. Mrs.ARCHANA MADAM. FIXATION. It is the process by which the morphology and . microscopic anatomy of the tissue is preserve as. Tissue Processing. Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. Techniques. By. DR ANYANWU GE. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . between chambers and the stream reach. Hilary L. Madinger, Robert O. Hall. University of Wyoming. nitrogen fixation. N. 2. NH. 3. Organic C. photosynthesis. CO. 2. Low N . ecosystems. Nitrogen fixation requires energy input as carbon from photosynthesis. Light and Electron Microscopes. Tissue Preparation. E-mail: . a.al-nuaimi@sheffield.ac.uk. E. mail: abdulameerh@yahoo.com . Prof. . Abdulameer. Al-. Nuaimi. Histology :. Is a . branch of anatomy that deals with the minute structure of animal and plant . THE GENERAL VISCRA OF THE RAT. Definition of Fixation . In the fields of . histology. , . pathology. , and . cell biology. , . fixation. is a . chemical process. by which . biological tissues. are . Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. 10% NEUTRAL BUFFERED FORMALIN. Sodium phosphate, monobasic 4.0 gm . Sodium phosphate, dibasic 6.5 gm . Formaldehyde, 37% 100.0 ml . Distilled water 900.0 ml. Glutaraldehyde. It gives very good overall cytoplasmic and nuclear detail . Technicalmethods.w..erA+FigurePositivemyeloperoxidasereactionwith2,7-FDAassubstrateinacaseofacutemyeloidleukaemia(M2j.NoteapositiveAuerrodintwoblasts(x1400).QUANTITATIVERESULTSThepercentagesofpositive Fixation is an attempt at stabilizing biological systems for viewing in a vacuum with minimal distortion to their cytomorphology and cytochemistry. Biological electron microscopy generally employs t It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. . . . . 10. % Neutral Buffered Formalin is the routine fixative. . Tissue Processing. Tissue processing consists of 3 steps. :. . 1-. Dehydration . . 2-. Clearing. . . Molecular nitrogen (N2) . is a very common chemical compound in which two nitrogen atoms are tightly bound together. . The . strong triple-bond between the atoms in molecular nitrogen makes this compound difficult to break apart, and thus nearly . Embedding. Sectioning. Staining. Fixation. It is important to . maintain. cells in as life-like a state as possible . to prevent post-mortem changes as a result of putrefaction (destruction of tissue by bacteria or fungi) and autolysis (destruction of tissue by its own enzymes). .