PPT-Procedures Fixation Tissues must be immersed in fixative immediately after removal from

10 Neutral Buffered Formalin is the routine fixative Tissue Processing Tissue processing consists of 3 steps 1 Dehydration 2 Clearing . Prepared by : Reem Aldossari. THE GENERAL VISCRA OF THE RAT. Definition of Fixation . In the fields of . histology. , . pathology. , and . cell biology. , . fixation. is a . chemical process. by which . TOPIC:. FIXATION. SUBMITTED BY. RINSEENA M. GAFOOR. SUBMITTED TO. Mr. REJO JACOB JOSEPH. &. Mrs.ARCHANA MADAM. FIXATION. It is the process by which the morphology and . microscopic anatomy of the tissue is preserve as. Tissue Processing. Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. Techniques. By. DR ANYANWU GE. Introduction. Histological technique deals with the preparation of tissue for microscopic examination. . The aim of good histological technique to preserve microscopic anatomy of tissue. . Light and Electron Microscopes. Tissue Preparation. E-mail: . a.al-nuaimi@sheffield.ac.uk. E. mail: abdulameerh@yahoo.com . Prof. . Abdulameer. Al-. Nuaimi. Histology :. Is a . branch of anatomy that deals with the minute structure of animal and plant . THE GENERAL VISCRA OF THE RAT. Definition of Fixation . In the fields of . histology. , . pathology. , and . cell biology. , . fixation. is a . chemical process. by which . biological tissues. are . Objective. s. What is . tissue processing. ?. What are the . major steps . in tissue processing?. Identify . types of fixative. .. The . advantage of each fixative. .. The . disadvantage of each fixative. 10% NEUTRAL BUFFERED FORMALIN. Sodium phosphate, monobasic 4.0 gm . Sodium phosphate, dibasic 6.5 gm . Formaldehyde, 37% 100.0 ml . Distilled water 900.0 ml. Glutaraldehyde. It gives very good overall cytoplasmic and nuclear detail . . Intra operatively. Haemorrhage. Fractured root apex. Damage to adjacent tooth. Damage to adjacent soft tissues. Fractured mandible. Collapse in the dental chair. Post operatively. Postoperative instructions. Fixation is an attempt at stabilizing biological systems for viewing in a vacuum with minimal distortion to their cytomorphology and cytochemistry. Biological electron microscopy generally employs t It is the branch of science which deals with the gross & microscopic study of normal tissue. Histopathology:. It is the branch of science which deals with the gross & microscopic study of tissue affected by disease.. . Tissues have two interacting components:. Cells .. . Extracellular matrix (ECM).which is consist of many kinds of macromolecules.. The (ECM) function :. Supports the cells and the fluids that transports nutrients to the cells .. These techniques included:. *Anesthetization. *Fixation. *Embedding. *cutting. *mounting onto slides. *Staining. *Washing. *mounting . *preservation. Animal experiments are necessary for a better understanding of diseases and for developing new therapeutic strategies. Animal experiment include Mouse, Rat, Rabbit, Hamster,..... Embedding. Sectioning. Staining. Fixation. It is important to . maintain. cells in as life-like a state as possible . to prevent post-mortem changes as a result of putrefaction (destruction of tissue by bacteria or fungi) and autolysis (destruction of tissue by its own enzymes). .