PPT-Microbial kinetics of growth and substrate utilization.

Batch culture and Kinetics of Microbial growth in batch culture After inoculation the growth rate of the cells gradually increases The cells grow at a constant maximum rate and this period is known as the . CLS 212: . Medical . Microbiology. Factors Affecting Microbial Growth. There . are some factors that affect and control the growth of microorganisms around us, in hospitals, in the laboratory, and in industrial settings. . Reaction Velocity = A e. -. Ea. /RT. where,. A = pre-exponential factor, or y-. intercept. Ea. = activation energy of the substrate. R = universal gas constant. T = temperature, . o. K. Boone et al (2003) Nature 396:570-572.. INTRODUCTION. GROWTH CURVE. LAG PHASE . EXPONENTIAL PHASE. STATIONARY PHASE. DECLINE PHASE. . INDEX. A closed culture system which contains initial and limited amount of nutrients.. The inoculated culture undergoes 4 phases i.e . Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Microbial growth is the increase in number of cells, not cell size. Microbial Growth. The Requirements for Growth: . Physical Requirements. Temperature. Minimum growth temperature. Optimum growth temperature. Christian Kendall and Galen Mack-Crane. Reactions. Mass-Action Kinetics. Michaelis and Menten (1913). . First Order. Time (years). [C] conc. by %mass. Second Order Unimolecular. Enzyme-Substrate Reactions. Lipitor: HMG-CoA reductase, inhibits a liver enzyme that is important in biosynthesis of cholesterol (>$100 billion total sales since 1996).. Viread & Emtriva: reverse transcriptase inhibitors, anti-retrovirus (HIV).. 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all 1. Microbial survival strategies. 2. Microbial growth. 3. Effects of the environment. 4. Microbial cultures. 5. Growth measurements. 6. Antimicrobial agents. 7. Antibiotics. 1. . Microbial. . survival. 1. Temperature. 2. . PH. 3. . Water activity. 4. . Oxygen and redox. Temperature. Minimum Temperature. . The temperature below which no growth occurs.. At temperature below the minimum the properties of cell membranes change so that they can no longer transport materials into the cell. Scientific opportunities with . als. -U. 1. Damage-free probing of aerosol reactions with <1 . m. s time resolution. Probe with soft x-ray . nanoprobes. , full-field soft x-ray microscopy. Aerosols are submicron compartmentalized chemical reactors. biotechnology.important. branch of Microbiology where economically valuable products are produced from cheaper raw materials by using large culture of . m.o.. Up stream and Downstream process.. Screening-Definition.. Microbiology. Outline. Microbial growth . in . natural environments. . Measurement . of . microbial population size. Principles of . microbial control. . Physical, chemical and biological control. . Kinetics. Growth Curve. Log CFU/ml. Optical Density. Lag. First Order Kinetics. Food microbiology is concerned with all phases. Of microbial growth (. lag,log. , stationary, death phase).Growth curves are normally plotted as the number of cells on a log scale or log10 cell number versus time..