PPT-Microbial kinetics of growth and substrate utilization.

Batch culture and Kinetics of Microbial growth in batch culture After inoculation the growth rate of the cells gradually increases The cells grow at a constant maximum rate and this period is known as the . C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. Reaction Velocity = A e. -. Ea. /RT. where,. A = pre-exponential factor, or y-. intercept. Ea. = activation energy of the substrate. R = universal gas constant. T = temperature, . o. K. Boone et al (2003) Nature 396:570-572.. Closed Cultivation Systems. Open Cultivation Systems. Semi-Open Cultivation Systems. . Closed Cultivation Systems. Operates in a closed system.. All materials are added to the system at the start of the process, the system is then closed and products removed only when the process is complete.. A. Microbial Reproduction. 1. binary (transverse) fission. A) parent cell enlarges and duplicates all its genetic material. B) DNA copies move to opposite ends of parent and attach to a section of the cell membrane as it begins to pinch together at the center. INTRODUCTION. GROWTH CURVE. LAG PHASE . EXPONENTIAL PHASE. STATIONARY PHASE. DECLINE PHASE. . INDEX. A closed culture system which contains initial and limited amount of nutrients.. The inoculated culture undergoes 4 phases i.e . Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. In the Environment. Table 9.1 Terminology of Microbial Control. Figure 9.1 A plot of microbial death rate.. Basic Principles of Microbial Control. Action of Antimicrobial Agents. Alteration of cell walls and membranes. Microbiology. Microbial Growth. Microbial requirements for growth:. 1. . Physical. A. . Temperature. Optimal growth temperature. Permissible range. human pathogens optimal = . 37°C. Microbial Growth. . Substrate. . utilization. in . Fermentation. Batch culture and Kinetics of Microbial growth in batch culture. After inoculation the growth rate of the cells gradually increases.. The cells grow at a constant, maximum, rate and this period is known as the . Musafer. PhD. Microbial growth defined as an increase in cellular constituents resulting an increase in a microorganism size, population number or both.. 1-Lag phase. When M.O are inoculated into fresh culture medium, will not reproduce immediately, therefore this period is called the lag phase. . Growth: . A. n . increase in cell number, not in cell size. .. Bacteria . grow and divide by binary fission, a rapid and relatively simple process. .. Phases . of Growth Bacterial Growth Curve : . When . biotechnology.important. branch of Microbiology where economically valuable products are produced from cheaper raw materials by using large culture of . m.o.. Up stream and Downstream process.. Screening-Definition.. Microbiology. Outline. Microbial growth . in . natural environments. . Measurement . of . microbial population size. Principles of . microbial control. . Physical, chemical and biological control.