PPT-MICROBIAL GROWTH KINETICS

INTRODUCTION GROWTH CURVE LAG PHASE EXPONENTIAL PHASE STATIONARY PHASE DECLINE PHASE INDEX A closed culture system which contains initial and limited amount of nutrients The inoculated culture undergoes 4 phases ie . CLS 212: . Medical . Microbiology. Factors Affecting Microbial Growth. There . are some factors that affect and control the growth of microorganisms around us, in hospitals, in the laboratory, and in industrial settings. . in vitro. CLS 212: Medical Microbiology. . Microbicidal. or . Microbistatic. ?. . Microbicidal. . Microbicidal. . is . the process . or . an agent . that . kills. the microorganism. . Batch culture and Kinetics of Microbial growth in batch culture. After inoculation the growth rate of the cells gradually increases.. The cells grow at a constant, maximum, rate and this period is known as the . A. Microbial Reproduction. 1. binary (transverse) fission. A) parent cell enlarges and duplicates all its genetic material. B) DNA copies move to opposite ends of parent and attach to a section of the cell membrane as it begins to pinch together at the center. In the Environment. Table 9.1 Terminology of Microbial Control. Figure 9.1 A plot of microbial death rate.. Basic Principles of Microbial Control. Action of Antimicrobial Agents. Alteration of cell walls and membranes. Microbiology. Microbial Growth. Microbial requirements for growth:. 1. . Physical. A. . Temperature. Optimal growth temperature. Permissible range. human pathogens optimal = . 37°C. Microbial Growth. Microbial growth is the increase in number of cells, not cell size. Microbial Growth. The Requirements for Growth: . Physical Requirements. Temperature. Minimum growth temperature. Optimum growth temperature. . Substrate. . utilization. in . Fermentation. Batch culture and Kinetics of Microbial growth in batch culture. After inoculation the growth rate of the cells gradually increases.. The cells grow at a constant, maximum, rate and this period is known as the . Musafer. PhD. Microbial growth defined as an increase in cellular constituents resulting an increase in a microorganism size, population number or both.. 1-Lag phase. When M.O are inoculated into fresh culture medium, will not reproduce immediately, therefore this period is called the lag phase. . Growth: . A. n . increase in cell number, not in cell size. .. Bacteria . grow and divide by binary fission, a rapid and relatively simple process. .. Phases . of Growth Bacterial Growth Curve : . When . 1. Microbial survival strategies. 2. Microbial growth. 3. Effects of the environment. 4. Microbial cultures. 5. Growth measurements. 6. Antimicrobial agents. 7. Antibiotics. 1. . Microbial. . survival. biotechnology.important. branch of Microbiology where economically valuable products are produced from cheaper raw materials by using large culture of . m.o.. Up stream and Downstream process.. Screening-Definition.. 9. Figure 9.1 A plot of microbial death rate.. Constant percentage. of the extant population. is killed each minute. Basic Principles of Microbial Control. Action of Antimicrobial Agents. Alteration of cell walls and membranes. Microbiology. Outline. Microbial growth . in . natural environments. . Measurement . of . microbial population size. Principles of . microbial control. . Physical, chemical and biological control.