MCB 317 Topic 10 part 3 A Story of Transcription Why are Acitvators cartooned as two balls held together by a tether Coactivators amp chromatin remodeling complexes not shown General Factors ID: 1047307
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1. MCB 317Genetics and GenomicsMCB 317 Topic 10, part 3A Story of Transcription
2. Why are Acitvators cartooned as two balls held together by a tether?Co-activators & chromatin remodeling complexes not shown
3. General FactorsRNAP ActivatorsProperties of Activators
4. ActivatorsMultiple functions = multiple domainsDNA binding domainActivation domainDimerization domain (some)Ligand binding domain (some)
5. Genes in Yeast vs. Larger Euk
6. Reporter gene in yeast -> use to study activators
7. Deletion Analysis of Coding Region -> Truncated Proteins
8. Domains of Activators
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10. Properties of Activators:Transcription factor families
11. Gene FamiliesIt is more common to create variations of a useful protein structure than it is to create a new structureFamilies created by gene duplication and divergence
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15. Gene Families are Generated by Duplication and Divergence
16. ActivatorsMultiple functions = multiple domainsDNA binding domainActivation domainDimerization domain (some)Ligand binding domain (some)
17. Helix-turn-helixHelix-loop-helixHomeoboxZinc fingerLeucine zipperActivators classified based on their DNA binding domain: Transcription factor families
18. Activators classified based on their DNA binding domain: Transcription factor familiesDifferent Family Members = Different Binding SiteProtein Domains andStructural Motifs
19. Protein – DNA interactionGeneral Properties of Proteins that bind DNA
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25. Helix-turn-helix
26. Homeodomain
27. Zinc finger type I structure
28. LeucineZipperActivators (factors) that dimerize only bind DNA as dimers
29. Homodimers bind inverted repeats
30. Homodimers, Heterodimers, Family Members and Recognition Sites
31. Zinc-finger type II Heterodimers
32. Helix-loop-helix dimer
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35. Cell Type Specific Patterns of Txn = Combination of Which Receptors and Txn Factors (Activators) a Cell Produces
36. Some Key Activators -> Txn of other Activators
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38. ActivatorsMultiple functions = multiple domainsDNA binding domainActivation domainDimerization domain (some)Ligand binding domain (some)
39. Gene family membersGene GeneGeneOrthologParalogOrthologs = family members in different speciesParalogs = family members in same species
40. Blast SearchYeast = 34 Zinc Finger Txn Factors (Activators)
41. AbProteinExpressionPatternGeneGene (Organism 2)ParalogsMutant GeneBiochemistryGeneticsMutant Organism1234785691011Orthologs and Paralogs9
42. Many Types of Steroid Hormones
43. A Variety of Family Members of Steroid-binding Txn Factors (Activators)In General Family Members have similar properties:Monomer v. dimer, mode of activation/inhibition, even types of genes regulated: homeodomains -> genes invovled in development
44. Gene FamiliesIt is more common to create variations of a useful protein structure than it is to create a new structureFamilies created by gene duplication and divergence