PPT-Welcome BIO510 Recombinant DNA Techniques Lab

Dr Brian Rymond Instructor amp Christen Wanstrath TA Make and break DNA and RNA in a variety of ways and test the consequences in the host organism E coli S cerevisiae C elegans. Recombinant DNA Technology. T. echnique that allows DNA to be . combined. from different sources. DNA code universal. Foundation of genetic engineering. Review. What are enzymes? What function do they serve?. Objective. Students will model the process of using restriction enzymes and plasmids to form recombinant DNA.. Background Information. major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes.. Synthetic . Nucleic Acid Molecules . (NIH Guidelines). Northern Arizona University. Office of Regulatory Compliance. Shelley Jones, Director of Biological Safety. Shelley.Jones@nau.edu. 928-523-7268. . Hameed. Abdullah. (. or . rDNA. ) is made by combining DNA from two or more sources. In practice, the process often involves combining the DNA of different organisms. . The . process depends on the ability of cut, and re-join, DNA molecules at points identified by specific sequences of nucleotide bases called restriction sites. DNA fragments are cut out of their normal position in the chromosome using . Definition. Steps . Applications. INTRODUCTION. Recombinant DNA.  (. rDNA. ): .  . DNA.  molecules formed by laboratory methods of . genetic recombination.  (such as . molecular cloning. ) to bring together genetic material from multiple sources, creating . BIOTECHNOLOGY. What. . is. . biotechnology. ? . Biotechnology. = . bios. (. life. ) + logos (. study. . of). Literally. ‘. the. . study. of . tools. . from. living . things. ’. What. . www.edvotek.com. EDVOTEK. The . Biotechnology Education. Company. Celebrating OVER 30 years . of science education!. Construction & Cloning of Recombinant DNA. From Campbell Biology, . Chp. . 20. Concept and basic steps in recombinant DNA technology and gene cloning. -2-. Recombinant DNA technology is playing a vital role in improving health conditions by . developing new vaccines and pharmaceuticals. 683 Human Erythropoietin (hEPO) is the main hormone involved in the differentiation, proliferation and maintaining of physiologic levels of erythroid stem cells, it was also the rst hematopoietic Technology. . and. . Biotechnology. II. . FE314. -. . Biotechnology. Spring . 201. 6. Some. . applications. of . recombiant. DNA . technology. in . Biotechnology. *. Diabates-Insulin. . production. Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria . Presentations. INTRODUCTION, PURPOSE, METHODS, RESULTS, CONCLUSIONS, REFERENCES.. First to isolate DNA. Fredrick . Meischer. (1868). 1950’s: What is the material of heredity?. Protein? . DNA?. Martha Chase, Alfred Hershey. Dr.V. S. Bheemareddy. Following . steps are involved in genetic engineering. .. Isolation of desired gene or DNA.. Construction of recombinant DNA. Insertion of recombinant DNA in to host cells. Selection of transformed cells or organisms. Profit. What the heck is recombinant DNA?. Recombinant DNA is what you get when you combine DNA from two different sources.. For Example:. Mouse + Human DNA. Human + Bacterial DNA. Viral + Bacteria DNA.