PPT-Discovery of a new type of bacterial enzyme able to cleave bonds in lignin

Kontur W S et al A heterodimeric glutathione S transferase that stereospecifically breaks lignins βRaryl ether bond reveals the diversity of bacterial β etherases . th. 2010. PBIO 691: [Plant Cell Walls]. An aromatic heteropolymer. Monomers Synthesized from Phenylalanine in the cytoplasm . Deposition of lignin occurs both within the secondary cell wall and in the middle lamella. Topic 2: . Enzyme. . Kinetics. What is an Enzyme?.  . Enzymes: .  are biological molecules (proteins) that act as . catalysts*.  and help complex reactions . take place. Basically, they . break a protein down into its amino . Approach. for . Phenolics. Lignin . Pyrolysis for the Production of Phenolic . Compounds. Bio-based industries, such as pulp- and paper and . lignocellulosic. . biorefineries. , produce lignin as a main by-product. Although lignin is a major renewable source of aromatic chemicals, it is mainly combusted for heat and power. ECN has developed LIBRA, a lignin . RESEARCHThe natural catalytic function of31CuGE glucuronoyl esterase in31hydrolysis of31genuine lignincarbohydrate complexes from31birchCaroline Mosbech Jesper Holck Anne S Meyer and Jane Wittrup Agge prostanoids. in the body.. These . prostanoids. including prostaglandins, prostacyclin and thromboxane . , they are . important biological mediators that play . important . role in the development of pain and inflammation in the body. So it is possible to get relief from pain and inflammation by inhibiting the COX enzyme.. nickel(II) and cadmium(II) ions removal. Tijana . Stanišić. , Ana . Popović. , Jelena . Rusmirović. , Maja . Đolić. , Mirjana . Ristić. , . . Aleksandra . Perić-Grujić. and Aleksandar . Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. DNA manipulating enzymes:. Oates, N.C., . Abood. , A., . Schirmacher. , A.M., Alessi, A.M., Bird, S.M., Bennett, J.P., Leadbeater, D.R., Li, Y., . Dowle. , A.A., Liu, S., . Tymokhin. , V.I., Ralph, J., McQueen-Mason, S.J., Bruce, N.C., “. Restriction enzymes . Restriction enzymes . (or restriction endonucleases), are bacterial enzymes that cleave both strands of DNA at or near specific recognition nucleotide sequences (4 -8 bp ) known as restriction sites. . Tsuji Y., . Vanholme. R., . Tobimatsu. Y., Ishikawa Y., Foster C. E., . Kamimura. N., . Hishiyama. S., Hashimoto S., Shino A., Hara H., Sato-Izawa K., . Oyarce. P., . Goeminne. G., . Morreel. K., Kikuchi J., Takano T., Fukuda M., Katayama Y., . Presented . by. Ms. . P. . . H. . Giri. Department of Microbiology. Deogiri . College, Aurangabad. B.Sc. T. Y. . Semester VI. Paper No. XIX. Recombinant DNA Technology. Ms. . Priyanka. H. . Giri. Steps in RDT:. Kontur. , W. S. . et al.. . “. Novosphingobium. . aromaticivorans. uses a Nu-class glutathione . S. -transferase as a glutathione . lyase. in breaking the β-aryl ether bond of lignin.” . Journal of Biological Chemistry . 1. S.S. Macdonald, J.H. Pereira, F. Liu, G. Tegl, . A. DeGiovanni, J.F. Wardman, S. Deutsch, Y. Yoshikuni, P.D. Adams, and S.G. Withers, . ACS Cent. Sci.. . 8. , 430 (2022), doi:10.1021/acscentsci.1c01570. Culture Test. What is Bacterial culture. Culture media . :. Is a method of multiplying microbial organisms by letting them produce in culture media under controlled laboratory conditions . . Bacteria like any living cell they need organic and inorganic materials for their live(water, .