PPT-Topic 2: Enzyme Kinetics

Topic 2 Enzyme Kinetics What is an Enzyme   Enzymes  are biological molecules proteins that act as  catalysts  and help complex reactions take place Basically they break a protein down into its amino . C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. Lecture 8: Tuesday, 9/15/15. Today’s Objective. Kinetics of rectilinear motion. . What is Kinetics?. Kinetics is the study of the relations between unbalanced forces and the resulting changes in . Lecturer Dr. . Kamal. E. M. . Elkahlout. Assistant Prof. of . Biotechnology. 1. CHAPTER 3. Immobilized . enzymes and their . uses. 2. Enzyme reactors. An enzyme reactor consists of a vessel, or series of vessels, used to perform a desired conversion by enzymatic means (Figure 5.1). . Kinetics Purpose: 1) Investigate the kinetics of LDH purified from bovine heart and muscle 2) Learn how to determine kinetic information 3) Understand the effects of inhibitors on enzyme activity En 10-1 :. Munich, Anne, Fluke, and Yu. Factors Affecting . Enzyme Activity. We are doing this experiment in order to test and find out the factors that has affects on the enzyme. . The indicator tells the substance’s chemical characteristic(pH). For red cabbage indicator. (. Danio. . rerio. ) . as a model. Chris A. . McCabe. 1. , Chris W. Theodorakis. 2. , Theodore B. Henry. 1. and Mark G. J. . Hartl. 1. Introduction . Environmental stressors can damage the DNA of aquatic organisms. MAT 493. 5/6/2015. Outline. Introduction. Homogeneous Case. Non-Homogeneous Case. Semi-Linear Case. Introduction. What is a Chemical Reaction?. A process that transforms one or more substances into another.. E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. In this experiment, we will continue to study acid phosphatase kinetics.. Objective:. To establish the relationship between enzyme concentration and the rate of an enzyme catalyzed reaction.. The reaction rate will increase as the concentration of enzymes is increased but there must be a . Therapy of enzyme defects: general considerations. How many organs are affected by the enzyme defect: One organ, a few, or all organs?. How severe is the defect?. Can the defect be adequately controlled by conventional treatment?. Lipitor: HMG-CoA reductase, inhibits a liver enzyme that is important in biosynthesis of cholesterol (>$100 billion total sales since 1996).. Viread & Emtriva: reverse transcriptase inhibitors, anti-retrovirus (HIV).. 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all Scientific opportunities with . als. -U. 1. Damage-free probing of aerosol reactions with <1 . m. s time resolution. Probe with soft x-ray . nanoprobes. , full-field soft x-ray microscopy. Aerosols are submicron compartmentalized chemical reactors. 1. Enzyme concentration. 2. Temperature. 3. Hydrogen ion concentration or pH. 4. Substrate concentration. 5. Inhibitors. 6. Product concentration. 7. Activators. 8. Physical agents. Factor affecting enzyme kinetics.