PPT-Topic 2: Enzyme Kinetics

Topic 2 Enzyme Kinetics What is an Enzyme   Enzymes  are biological molecules proteins that act as  catalysts  and help complex reactions take place Basically they break a protein down into its amino . 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all C483 Spring 2013. Questions. 1. Enzymes . that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called. A. ) . isomerases. .. B. ) . lyases. .. C. ) ligases.. (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 15. Lecture . 15 . – . Tuesday 3/12/2013. Enzymatic Reactions. Kinetics Purpose: 1) Investigate the kinetics of LDH purified from bovine heart and muscle 2) Learn how to determine kinetic information 3) Understand the effects of inhibitors on enzyme activity En Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Pratt & . Cornely. . Ch. 7. Enzyme Kinetics. How fast an enzyme catalyzed reaction goes. Why study enzyme kinetics?. Helps us understand mechanism of enzyme (how it works). Investigation of mutations in metabolic pathways. Biochemistry. , 6th . Edition, © . 2016 . by Oxford University Press. Online Video. How Enzymes Work. Section 6.1, page 192. From McKee and McKee, . Biochemistry. , 6th . Edition, © . 2016 . by Oxford University Press. (CRE) is the field that studies the rates and mechanisms of chemical reactions and the design of the reactors in which they take place.. Lecture. 15. Lecture . 15 . – . Tuesday. Enzymatic Reactions. MAT 493. 5/6/2015. Outline. Introduction. Homogeneous Case. Non-Homogeneous Case. Semi-Linear Case. Introduction. What is a Chemical Reaction?. A process that transforms one or more substances into another.. E S <=> ES <=> ES* <=> EP <=> E P. Michaelis-Menten Kinetics. E S <=> ES <=> ES* <=> EP <=> E P. Rate of Formation. Substrate. Product. Enzyme. Enzyme-. Christian Kendall and Galen Mack-Crane. Reactions. Mass-Action Kinetics. Michaelis and Menten (1913). . First Order. Time (years). [C] conc. by %mass. Second Order Unimolecular. Enzyme-Substrate Reactions. Lipitor: HMG-CoA reductase, inhibits a liver enzyme that is important in biosynthesis of cholesterol (>$100 billion total sales since 1996).. Viread & Emtriva: reverse transcriptase inhibitors, anti-retrovirus (HIV).. 1 In this exercise we will look at the catalytic behavior of enzymes. You will use Excel to answer the questions in the exercise section. At the end of this session, you must hand in answers to all 1. Enzyme concentration. 2. Temperature. 3. Hydrogen ion concentration or pH. 4. Substrate concentration. 5. Inhibitors. 6. Product concentration. 7. Activators. 8. Physical agents. Factor affecting enzyme kinetics.