PPT-Topic- Proteins Presented

by Ms P H Giri Department of Microbiology Deogiri College Aurangabad BSc F Y Semester II Paper No V Basic Biochemistry Unit 3 Proteins Ms Priyanka H Giri CLASSIFICATION OF PROTEINS . B. Suarez, R. Martinez, O. Diaz, H. Jones, T. Ashraf, E. Priddis, K. Durham, Undergraduate Biology Research, Cochise Community College, Sierra Vista, AZ . INTRODUCTION.  . Genomic and proteomic studies can reveal multi-dimensional aspects of biological model organisms. DNA sequencing and short tandem repeats are utilized to characterize organism’s phylogenetic relationships; another approach is to study their various proteins. Many genomic studies utilize extraction and amplification of nucleic acids to help make detection more straightforward. There is no proteomic procedure similar to PCR that would identify proteins at their naturally existing concentration, as well as the presence of many other proteins for comparative studies. Most methods for studying proteins revolve around running 1D, 2D, or 3D gels, and comparing and identifying similar proteins. . Introduction:. Plasma proteins are:. Serum albumin.. Serum globulin.. Fibrinogen.. Serum contains albumin and globulin.. Fibrinogen is absent . Converted into fibrin during blood clotting.. Therefore albumin and globulin are called serum albumin and serum globulin.. . from lack of structure to. pleiotropy. of functions. Lilia Iakoucheva. University of California, San Diego. Ordered Proteins. Disordered Proteins. Uversky and Dunker, 2012, Anal . Chem. Outline. The 20 different amino acids. 7.5.1: Explain the four levels of protein structure, indicating the significance of each level.. Peptide bonds link the amino acids together . Polypeptide with five amino acids. . Protein. The . main function of protein is to . build and repair tissues . (muscle tissue). Protein. Protein can be a source of energy but should be used as a last resort. CHAPTER 3, Part 1 . Amino Acids and Peptides . To know the structure and naming of all 20 protein amino acids. To know the structure and properties of peptides and the particularly the structure of the peptide bond.. Levels of Protein Structure. Primary 1º Structure. The primary structure is simply the sequence of amino acids in a protein.. Chains of amino acids are written from the amino terminus (N-terminus) to the carboxyl terminus (C-terminus).. B.2. Properties of 2-amino acids . (B.2.2). Zwitterion. (dipolar) . amino acids contain both acidic and basic groups in the same molecule . therefore, are . amphoteric. in nature (capable of behaving as acids or bases). Dialysis. (. Isolation Of Lactate Dehydrogenase Enzyme . ). BCH 333 [practical]. Objective:. 1-To . learn the technique of isolation of proteins on the basis of their solubility. . salting . in, salting out of proteins . Proteins account for more than 50% of the dry mass of most cells. Protein functions include structural support, storage, transport, cellular communications, movement, and defense against foreign substances. Choosing a Good Topic. What Happens After Brainstorming Topics?. Strategies for Brainstorming an EDD Topic. Brainstorming Review. What Do You Know?. What Do You Love?. Don’t You Hate It When . . .. Amino . Acids. In a transamination reaction, . aspartate transaminase (AST) . catalyzes the reversible transfer of an amino group between glutamate and aspartate.. an . α. -amino group is transferred from an amino acid to an . 4. calories per gram. . The main function of protein is to . build and repair body tissues. .. If carbohydrates and fat are not available, your body will use protein. Is this a good thing? . You must eat protein . Unit 1 Advanced Higher. Miss Aitken. Pages 16-25. Textbook. Proteomics. Genome:. complete set of DNA. Comprises of coding and non-coding areas. 20-25000 genes in human genome. Proteome:. Entire set of proteins that can be expressed from a genome.