PDF-Journal of Chromatography A 1186 (2008) 51-66 Publisher: ELSEVIER SC

Buser2347467coverDate042F042F2008alid1140654144rdoc7fmthighorigsearchcdi5248sort rdocanchorviewcct23acctC000057013version1urlVersion0userid2347467md5 11a. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with Copyright © 2012, 2008, 2004, 2000, 1996, 1993 by Saunders, an affiliate of Elsevier Inc. . External Anatomy. Bony orbital cavity surrounded by cushion of fat protects eye. Eyelids are like two movable shades that further protect eye from injury, strong light, and dust. GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann.